Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   PY718_RS11795 Genome accession   NZ_CP119011
Coordinates   2439032..2440525 (+) Length   497 a.a.
NCBI ID   WP_021513239.1    Uniprot ID   -
Organism   Escherichia coli strain CE0007a     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2434032..2445525
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY718_RS11775 (PY718_11770) pilD 2434680..2435489 (+) 810 WP_000895878.1 prepilin peptidase PppA Machinery gene
  PY718_RS11780 (PY718_11775) gspS2 2435555..2435965 (+) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  PY718_RS11785 (PY718_11780) gspC 2435983..2436942 (+) 960 WP_000135091.1 type II secretion system protein GspC -
  PY718_RS11790 (PY718_11785) gspD 2436972..2439032 (+) 2061 WP_000498830.1 type II secretion system secretin GspD -
  PY718_RS11795 (PY718_11790) pilB/pilB1 2439032..2440525 (+) 1494 WP_021513239.1 type II secretion system ATPase GspE Machinery gene
  PY718_RS11800 (PY718_11795) gspF 2440525..2441748 (+) 1224 WP_001173444.1 type II secretion system inner membrane protein GspF -
  PY718_RS11805 (PY718_11800) gspG 2441765..2442220 (+) 456 WP_001087289.1 type II secretion system major pseudopilin GspG -
  PY718_RS11810 (PY718_11805) gspH 2442224..2442787 (+) 564 WP_021513238.1 type II secretion system minor pseudopilin GspH -
  PY718_RS11815 (PY718_11810) gspI 2442784..2443155 (+) 372 WP_019842323.1 type II secretion system minor pseudopilin GspI -
  PY718_RS11820 (PY718_11815) gspJ 2443152..2443757 (+) 606 WP_021513237.1 type II secretion system minor pseudopilin GspJ -
  PY718_RS11825 (PY718_11820) gspK 2443754..2444731 (+) 978 WP_019842321.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55156.27 Da        Isoelectric Point: 6.0089

>NTDB_id=724734 PY718_RS11795 WP_021513239.1 2439032..2440525(+) (pilB/pilB1) [Escherichia coli strain CE0007a]
MVPVAQETSANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSSTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLAGVMAQRLVRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=724734 PY718_RS11795 WP_021513239.1 2439032..2440525(+) (pilB/pilB1) [Escherichia coli strain CE0007a]
ATGGTGCCTGTAGCACAGGAAACCTCCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGCCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAACGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAAGCTCGCCAGTTGATGGAAGATCTCGGTTCCGCCGAGGACTTCTTTACCCTCGCAGAAGAGCTGCCAGAAACGGAAGA
TTTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATTAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTAGACGGAACGCTACATGAAATGCTGCGT
CCCGGGCGCAAACTAGCCTCGCTGCTGGTGTCGCGTATCAAGGTGATGGCGCGGCTGGACATTGCCGAAAAGCGCGTGCC
GCAGGATGGACGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCTGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTGAGTCATGAACTGACC
GCGCAGTTGCGCCAGCTACTGCACAAACCGCACGGTATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCAGCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATTGCCGTCCAGGCATCGCTTACAGGGCACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTCGAGCCTTTCCTGC
TCTCTTCCAGTCTGGCGGGCGTGATGGCGCAGCGGCTGGTTCGCACGCTGTGCCCCGACTGCCGCCAGCCCGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGAACCGTCACTCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAACTGATTGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGTAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.372

100

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.765

85.513

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Legionella pneumophila strain ERS1305867

43.387

86.72

0.376
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilF Thermus thermophilus HB27

45

80.483

0.362