Detailed information NTG0723591

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: PWP91_RS12595
• Genome accession: NZ_CP118738
• Coordinates: 2499628..2500074 (-)
• Length: 148 a.a.
• NCBI ID: WP_032948129.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain P-1
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2494628..2505074

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PWP91_RS12560 (PWP91_12560)	-	2494746..2495123 (-)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-
PWP91_RS12565 (PWP91_12565)	-	2495328..2496194 (+)	867	WP_260317978.1	RluA family pseudouridine synthase	-
PWP91_RS12570 (PWP91_12570)	-	2496232..2497041 (-)	810	WP_014570791.1	metal ABC transporter permease	-
PWP91_RS12575 (PWP91_12575)	-	2497034..2497771 (-)	738	WP_058211243.1	metal ABC transporter ATP-binding protein	-
PWP91_RS12580 (PWP91_12580)	-	2497948..2498790 (-)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
PWP91_RS12585 (PWP91_12585)	-	2498787..2499224 (-)	438	WP_274998612.1	zinc-dependent MarR family transcriptional regulator	-
PWP91_RS12590 (PWP91_12590)	comGG	2499305..2499589 (-)	285	WP_017865155.1	competence type IV pilus minor pilin ComGG	Machinery gene
PWP91_RS12595 (PWP91_12595)	comGF	2499628..2500074 (-)	447	WP_032948129.1	competence type IV pilus minor pilin ComGF	Machinery gene
PWP91_RS12600 (PWP91_12600)	comGE	2500037..2500333 (-)	297	WP_250354455.1	competence type IV pilus minor pilin ComGE	Machinery gene
PWP91_RS12605 (PWP91_12605)	comGD	2500305..2500736 (-)	432	WP_012898621.1	competence type IV pilus minor pilin ComGD	Machinery gene
PWP91_RS12610 (PWP91_12610)	comGC	2500696..2500965 (-)	270	WP_023349160.1	competence type IV pilus major pilin ComGC	Machinery gene
PWP91_RS12620 (PWP91_12620)	-	2501462..2501662 (-)	201	WP_058203534.1	cold-shock protein	-
PWP91_RS12625 (PWP91_12625)	-	2502719..2502862 (-)	144	WP_014573124.1	hypothetical protein	-
PWP91_RS12630 (PWP91_12630)	-	2502938..2504410 (-)	1473	WP_274998613.1	ATP-binding protein	-
PWP91_RS12635 (PWP91_12635)	-	2504550..2504828 (-)	279	WP_274998614.1	hypothetical protein	-

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17111.91 Da
• Isoelectric Point: 8.6780

>NTDB_id=723591 PWP91_RS12595 WP_032948129.1 2499628..2500074(-) (comGF) [Lactococcus lactis strain P-1]
MERKLCNLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQDFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=723591 PWP91_RS12595 WP_032948129.1 2499628..2500074(-) (comGF) [Lactococcus lactis strain P-1]
ATGGAAAGGAAATTATGCAACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAGCAGGATTTTCTG
TATGTAACTAAAGACAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAGAGAACATTTATTTATCAATTTCCGGAAGATACGTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	75.54	93.919	0.709
comGF	Streptococcus mutans UA140	47.518	95.27	0.453
comGF	Streptococcus mutans UA159	46.809	95.27	0.446
comGF/cglF	Streptococcus mitis SK321	42.336	92.568	0.392
comGF/cglF	Streptococcus mitis NCTC 12261	42.336	92.568	0.392
comGF/cglF	Streptococcus pneumoniae Rx1	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae D39	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae R6	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae TIGR4	39.716	95.27	0.378