NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	M5T49_RS02185	Genome accession	NZ_CP103557
Coordinates	449522..450646 (+)	Length	374 a.a.
NCBI ID	WP_000228517.1	Uniprot ID	E2QFE2
Organism	Escherichia coli O25b:H4-ST131 strain 4993
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 444522..455646

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M5T49_RS02160 (M5T49_02160)	mscL	444639..445052 (-)	414	WP_000022450.1	large-conductance mechanosensitive channel protein MscL	-
M5T49_RS02165 (M5T49_02165)	trkA	445182..446558 (-)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
M5T49_RS02170 (M5T49_02170)	rsmB	446580..447869 (-)	1290	WP_000744765.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
M5T49_RS02175 (M5T49_02175)	fmt	447921..448868 (-)	948	WP_000004421.1	methionyl-tRNA formyltransferase	-
M5T49_RS02180 (M5T49_02180)	def	448883..449392 (-)	510	WP_000114984.1	peptide deformylase	-
M5T49_RS02185 (M5T49_02185)	dprA	449522..450646 (+)	1125	WP_000228517.1	DNA-protecting protein DprA	Machinery gene
M5T49_RS02190 (M5T49_02190)	smg	450618..451091 (+)	474	WP_000460673.1	DUF494 family protein Smg	-
M5T49_RS02195 (M5T49_02195)	yrdD	451120..451662 (+)	543	WP_001296461.1	type I DNA topoisomerase	-
M5T49_RS02200 (M5T49_02200)	tsaC	451667..452239 (+)	573	WP_001296460.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
M5T49_RS02205 (M5T49_02205)	aroE	452244..453062 (+)	819	WP_000451255.1	shikimate dehydrogenase	-
M5T49_RS02210 (M5T49_02210)	yrdB	453059..453316 (+)	258	WP_001070565.1	DUF1488 domain-containing protein	-
M5T49_RS02215 (M5T49_02215)	yrdA	453292..453846 (-)	555	WP_000005712.1	gamma carbonic anhydrase family protein	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40910.86 Da Isoelectric Point: 6.0395

                                >NTDB_id=723347 M5T49_RS02185 WP_000228517.1 449522..450646(+) (dprA) [Escherichia coli O25b:H4-ST131 strain 4993]

MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=723347 M5T49_RS02185 WP_000228517.1 449522..450646(+) (dprA) [Escherichia coli O25b:H4-ST131 strain 4993]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT

GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT

TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGTCTAAGTAAGGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	E2QFE2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.78	98.663	0.481
dprA	Vibrio cholerae strain A1552	49.355	82.888	0.409
dprA	Glaesserella parasuis strain SC1401	44.807	90.107	0.404
dprA	Haemophilus influenzae Rd KW20	44.214	90.107	0.398
dprA	Legionella pneumophila strain ERS1305867	44.242	88.235	0.39

Detailed information NTG0723347