Detailed information NTG0722727

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NX832_RS14465
• Genome accession: NZ_CP103413
• Coordinates: 2984748..2985866 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain AB2369
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2979748..2990866

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NX832_RS14455 (NX832_14455)	-	2982864..2983556 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
NX832_RS14460 (NX832_14460)	pilT	2983683..2984720 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
NX832_RS14465 (NX832_14465)	pilU	2984748..2985866 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NX832_RS14470 (NX832_14470)	fur	2985936..2986373 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
NX832_RS14475 (NX832_14475)	-	2986485..2986883 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
NX832_RS14480 (NX832_14480)	-	2986938..2987261 (-)	324	WP_000442587.1	RnfH family protein	-
NX832_RS14485 (NX832_14485)	-	2987261..2988328 (-)	1068	WP_002019572.1	hypothetical protein	-
NX832_RS14490 (NX832_14490)	-	2988481..2988936 (-)	456	WP_000782976.1	bacteriohemerythrin	-
NX832_RS14495 (NX832_14495)	-	2989189..2990037 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=722727 NX832_RS14465 WP_000347039.1 2984748..2985866(+) (pilU) [Acinetobacter baumannii strain AB2369]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=722727 NX832_RS14465 WP_000347039.1 2984748..2985866(+) (pilU) [Acinetobacter baumannii strain AB2369]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395