Detailed information NTG0722437

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: PWK65_RS00345
• Genome accession: NZ_CP118481
• Coordinates: 54214..55212 (+)
• Length: 332 a.a.
• NCBI ID: WP_002987726.1
• Uniprot ID: Q1JP25
• Organism: Streptococcus pyogenes strain 20185322
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 49214..60212

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PWK65_RS00325 (PWK65_00320)	-	49943..51283 (+)	1341	WP_002987721.1	hypothetical protein	-
PWK65_RS00330 (PWK65_00325)	purB	51652..52944 (+)	1293	WP_002987723.1	adenylosuccinate lyase	-
PWK65_RS00335 (PWK65_00330)	comR	53077..53988 (+)	912	WP_002986681.1	transcriptional regulator Rgg4/ComR	Regulator
PWK65_RS00340	comS	54072..54167 (+)	96	WP_198462994.1	quorum-sensing system DWW-type pheromone	Regulator
PWK65_RS00345 (PWK65_00335)	ruvB	54214..55212 (+)	999	WP_002987726.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
PWK65_RS00350 (PWK65_00340)	-	55350..55787 (+)	438	WP_002987727.1	low molecular weight protein-tyrosine-phosphatase	-
PWK65_RS00355 (PWK65_00345)	-	55811..56212 (+)	402	WP_002987730.1	MORN repeat-containing protein	-
PWK65_RS00360 (PWK65_00350)	-	56209..57984 (+)	1776	WP_002987732.1	acyltransferase family protein	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37585.14 Da
• Isoelectric Point: 5.1689

>NTDB_id=722437 PWK65_RS00345 WP_002987726.1 54214..55212(+) (ruvB) [Streptococcus pyogenes strain 20185322]
MARILDNDVMGNEEFSDRTLRPQYLREYIGQDKVKEQFAIFIEAAKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAVEKAGDLVAILNELEPGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPRIANRLLKRVRDYAQIIGDGIIT
AQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGGPVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQK
AYRHLGYPYQNT

Nucleotide

• Length: 999 bp

>NTDB_id=722437 PWK65_RS00345 WP_002987726.1 54214..55212(+) (ruvB) [Streptococcus pyogenes strain 20185322]
ATGGCTAGAATTTTAGACAATGATGTAATGGGAAATGAGGAGTTTTCGGATCGGACTCTTCGCCCTCAATATCTACGCGA
ATATATTGGACAAGATAAAGTTAAGGAACAATTCGCTATTTTTATAGAAGCAGCTAAAAGACGTGACGAATCTTTAGACC
ATGTTTTATTATTTGGACCTCCAGGACTGGGAAAAACAACAATGGCATTTGTGATTGCCAATGAATTGGGAGTTAATTTA
AAGCAAACTTCAGGTCCTGCCGTTGAGAAAGCTGGAGATTTGGTAGCTATTTTAAATGAATTAGAACCAGGGGATATTTT
GTTTATTGATGAAATTCACCGCATGCCAATGTCTGTTGAGGAAGTACTATATAGTGCTATGGAAGACTTCTATATTGATA
TTATGATTGGCGCTGGAGATACTAGTCGTAGTATTCATTTGGATCTGCCTCCATTTACATTGATTGGTGCTACTACACGT
GCAGGCATGTTATCTAATCCCCTCAGAGCTCGTTTTGGTATCACTGGTCACATGGAATACTATCAAGAAAAGGATTTGAC
AGAGATTGTGGAGCGTACAGCTACTATTTTTGAAATAAAAATAGACCATGAAGCTGCACGGAAATTAGCTTGTCGCAGTA
GAGGAACGCCCCGCATTGCTAATCGATTACTAAAACGAGTGCGTGACTATGCTCAAATTATTGGTGATGGCATCATAACG
GCTCAAATAACTGATAGAGCTTTGACTATGTTAGATGTTGACCGCGAAGGTCTCGATTACATTGATCAGAAAATTTTACG
AACGATGATTGAGATGTATCAGGGAGGACCAGTTGGTTTGGGGACATTATCTGTGAATATTGCCGAGGAGCGTAATACAG
TTGAAGAGATGTATGAACCCTACTTAATTCAAAAAGGCTTTCTCATGCGAACACGAACAGGTCGTGTAGCGACTCAAAAG
GCTTATCGTCATCTTGGCTATCCCTATCAAAACACTTAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• RuvB AAA lid domain (AAA_lid_4) (181-254)

3D structure

• AlphaFold DB: Q1JP25

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae R6	85.152	99.398	0.846
ruvB	Streptococcus pneumoniae D39	85.152	99.398	0.846
ruvB	Streptococcus pneumoniae TIGR4	84.848	99.398	0.843
ruvB	Bacillus subtilis subsp. subtilis str. 168	58.824	97.289	0.572
ruvB	Helicobacter pylori 26695	48.936	99.096	0.485
ruvB	Synechocystis sp. PCC 6803	50.16	94.277	0.473