Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NX062_RS12350 Genome accession   NZ_CP103328
Coordinates   2310240..2311310 (-) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis strain KC14-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2305240..2316310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NX062_RS12305 (NX062_12300) tapA 2305718..2306479 (-) 762 WP_014480251.1 amyloid fiber anchoring/assembly protein TapA -
  NX062_RS12310 (NX062_12305) yqzG 2306751..2307077 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  NX062_RS12315 (NX062_12310) spoIITA 2307119..2307298 (-) 180 WP_029726723.1 YqzE family protein -
  NX062_RS12320 (NX062_12315) comGG 2307370..2307744 (-) 375 WP_014480253.1 ComG operon protein ComGG Machinery gene
  NX062_RS12325 (NX062_12320) comGF 2307745..2308128 (-) 384 WP_046160582.1 ComG operon protein ComGF Machinery gene
  NX062_RS12330 (NX062_12325) comGE 2308154..2308501 (-) 348 WP_046160583.1 ComG operon protein 5 Machinery gene
  NX062_RS12335 (NX062_12330) comGD 2308485..2308916 (-) 432 WP_014480256.1 comG operon protein ComGD Machinery gene
  NX062_RS12340 (NX062_12335) comGC 2308906..2309202 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  NX062_RS12345 (NX062_12340) comGB 2309216..2310253 (-) 1038 WP_029317912.1 comG operon protein ComGB Machinery gene
  NX062_RS12350 (NX062_12345) comGA 2310240..2311310 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  NX062_RS12355 (NX062_12350) - 2311522..2311719 (-) 198 WP_046160584.1 CBS domain-containing protein -
  NX062_RS12360 (NX062_12355) corA 2311721..2312674 (-) 954 WP_015483432.1 magnesium transporter CorA -
  NX062_RS12365 (NX062_12360) yqhB 2312817..2314145 (+) 1329 WP_046160586.1 hemolysin family protein -
  NX062_RS12370 (NX062_12365) rsbRD 2314198..2315034 (-) 837 WP_015714260.1 RsbT co-antagonist protein RsbRD -
  NX062_RS12380 (NX062_12375) mgsR 2315258..2315638 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  NX062_RS12385 (NX062_12380) yqgY 2315870..2316115 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=721990 NX062_RS12350 WP_004399124.1 2310240..2311310(-) (comGA) [Bacillus subtilis strain KC14-1]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=721990 NX062_RS12350 WP_004399124.1 2310240..2311310(-) (comGA) [Bacillus subtilis strain KC14-1]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAAGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCAACTGGTTCAGGGAAGACTACCACACTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATTGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGTCGCCAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTTTACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGGAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1