Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NW957_RS07270 Genome accession   NZ_CP102977
Coordinates   1530041..1531015 (-) Length   324 a.a.
NCBI ID   WP_000697217.1    Uniprot ID   A0AAN2D5C1
Organism   Staphylococcus aureus strain 27-G-H     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1525041..1536015
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NW957_RS07230 (NW957_07230) gcvT 1525542..1526633 (-) 1092 WP_165468756.1 glycine cleavage system aminomethyltransferase GcvT -
  NW957_RS07235 (NW957_07235) - 1526792..1527316 (-) 525 WP_001015121.1 shikimate kinase -
  NW957_RS07240 (NW957_07240) - 1527306..1527452 (-) 147 WP_001789879.1 hypothetical protein -
  NW957_RS07245 (NW957_07245) comGF 1527549..1528046 (-) 498 WP_001829055.1 competence type IV pilus minor pilin ComGF Machinery gene
  NW957_RS07250 (NW957_07250) comGE 1527964..1528263 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  NW957_RS07255 (NW957_07255) comGD 1528250..1528696 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  NW957_RS07260 (NW957_07260) comGC 1528674..1528985 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  NW957_RS07265 (NW957_07265) comGB 1528999..1530069 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  NW957_RS07270 (NW957_07270) comGA 1530041..1531015 (-) 975 WP_000697217.1 competence type IV pilus ATPase ComGA Machinery gene
  NW957_RS07275 (NW957_07275) - 1531067..1531690 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  NW957_RS07280 (NW957_07280) - 1531687..1532016 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  NW957_RS07285 (NW957_07285) - 1532016..1533002 (-) 987 WP_000161314.1 ROK family glucokinase -
  NW957_RS07290 (NW957_07290) - 1532999..1533202 (-) 204 WP_000087561.1 YqgQ family protein -
  NW957_RS07295 (NW957_07295) - 1533183..1534646 (-) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  NW957_RS07300 (NW957_07300) - 1534658..1535197 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  NW957_RS07305 (NW957_07305) rpmG 1535406..1535555 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36869.90 Da        Isoelectric Point: 8.8516

>NTDB_id=721042 NW957_RS07270 WP_000697217.1 1530041..1531015(-) (comGA) [Staphylococcus aureus strain 27-G-H]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIAPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=721042 NW957_RS07270 WP_000697217.1 1530041..1531015(-) (comGA) [Staphylococcus aureus strain 27-G-H]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGCACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGTATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAACAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.691

100

0.997
  comGA Staphylococcus aureus N315

99.691

100

0.997