Detailed information NTG0072026

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: JQN53_RS02120
• Genome accession: NZ_AP024301
• Coordinates: 387978..388952 (-)
• Length: 324 a.a.
• NCBI ID: WP_011227913.1
• Uniprot ID: Q5SL87
• Organism: Thermus thermophilus strain HB5002
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 382978..393952

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JQN53_RS02100 (TthHB5002_04160)	-	383234..383662 (+)	429	WP_011227910.1	hypothetical protein	-
JQN53_RS02105 (TthHB5002_04170)	ftsH	383648..385504 (-)	1857	WP_203971616.1	ATP-dependent zinc metalloprotease FtsH	-
JQN53_RS02110 (TthHB5002_04180)	-	385577..386929 (+)	1353	WP_008631640.1	tetratricopeptide repeat protein	-
JQN53_RS02115 (TthHB5002_04190)	-	386950..387981 (-)	1032	WP_011172495.1	DUF4388 domain-containing protein	-
JQN53_RS02120 (TthHB5002_04200)	ruvB	387978..388952 (-)	975	WP_011227913.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
JQN53_RS02125 (TthHB5002_04210)	panB	388978..389763 (+)	786	WP_014630114.1	3-methyl-2-oxobutanoate hydroxymethyltransferase	-
JQN53_RS02130 (TthHB5002_04220)	-	389811..390737 (+)	927	WP_014630113.1	RluA family pseudouridine synthase	-
JQN53_RS02135 (TthHB5002_04230)	-	390734..391627 (+)	894	WP_203971619.1	site-specific DNA-methyltransferase	-
JQN53_RS02140 (TthHB5002_04250)	lysN	392265..393458 (-)	1194	WP_203971621.1	2-aminoadipate transaminase	-

Sequence

Protein

• Length: 324 a.a.
• Molecular weight: 35973.74 Da
• Isoelectric Point: 6.3232

>NTDB_id=72026 JQN53_RS02120 WP_011227913.1 387978..388952(-) (ruvB) [Thermus thermophilus strain HB5002]
MEDLALRPKTLDEYIGQERLKQKLRVYLEAAKARKEPLEHLLLFGPPGLGKTTLAHVIAHELGVNLRVTSGPAIEKPGDL
AAILANSLEEGDILFIDEIHRLSRQAEEHLYPAMEDFVMDIVIGQGPAARTIRLELPRFTLIGATTRPGLITAPLLSRFG
IVEHLEYYTPEELAQGVMRDARLLGVRITEEAALEIGRRSRGTMRVAKRLFRRVRDFAQVAGEEVITRERALEALAALGL
DELGLEKRDREILEVLILRFGGGPVGLATLATALSEDPGTLEEVHEPYLIRQGLLKRTPRGRVATELAYRHLGYPPPVGP
LLEP

Nucleotide

• Length: 975 bp

>NTDB_id=72026 JQN53_RS02120 WP_011227913.1 387978..388952(-) (ruvB) [Thermus thermophilus strain HB5002]
GTGGAAGACCTCGCCCTTAGGCCCAAGACCCTGGACGAGTACATCGGCCAGGAGCGCCTGAAGCAAAAGCTCCGGGTCTA
CCTCGAGGCGGCCAAGGCCCGAAAAGAACCCTTAGAGCACCTCCTCCTCTTCGGCCCCCCGGGCCTGGGCAAGACCACCC
TGGCCCACGTCATCGCCCACGAGCTTGGGGTCAACCTCCGGGTCACCTCCGGGCCCGCCATAGAGAAGCCTGGGGACCTC
GCCGCCATCCTGGCCAACTCCCTGGAGGAAGGGGACATCCTCTTCATTGACGAGATCCACCGCCTGAGCCGCCAGGCCGA
GGAGCACCTCTACCCCGCCATGGAGGACTTCGTCATGGACATCGTCATCGGCCAAGGTCCGGCGGCGAGGACCATCCGGC
TGGAGCTTCCCCGCTTCACCCTGATCGGGGCCACCACCCGGCCCGGCCTCATCACCGCGCCCCTCTTGAGCCGCTTCGGC
ATCGTGGAGCACCTGGAGTACTACACCCCCGAGGAGCTGGCCCAAGGGGTGATGCGGGACGCCCGCCTCCTCGGGGTGAG
GATCACGGAGGAGGCCGCCCTGGAGATCGGTAGGCGGAGCCGGGGCACCATGCGCGTCGCCAAGCGCCTCTTCCGGAGGG
TGCGGGACTTCGCCCAGGTGGCGGGGGAGGAGGTCATCACCCGGGAGCGGGCCTTGGAGGCCCTTGCCGCCCTGGGCCTG
GACGAGCTGGGCTTGGAGAAGCGGGACCGGGAGATCCTGGAGGTCCTCATCCTCCGCTTCGGCGGCGGCCCCGTGGGCCT
CGCCACCCTGGCCACCGCCCTCTCCGAGGACCCGGGCACCTTGGAAGAGGTGCACGAGCCCTACCTCATCCGCCAGGGCC
TTTTGAAGCGCACTCCCCGGGGCCGGGTGGCCACGGAACTGGCCTACCGCCACCTGGGCTACCCGCCCCCGGTGGGCCCC
CTCTTGGAGCCGTGA

Domains

Predicted by InterProScan.

• RuvB AAA lid domain (AAA_lid_4) (168-241)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (6-165)

• RuvB C-terminal winged helix domain (WHD_RuvB) (242-312)

3D structure

• PDB: 1HQC
• PDB: 1IXR
• PDB: 1IXS
• PDB: 8EFV
• PDB: 8EFY

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	59.105	96.605	0.571
ruvB	Streptococcus pneumoniae TIGR4	56.774	95.679	0.543
ruvB	Streptococcus pneumoniae R6	56.774	95.679	0.543
ruvB	Streptococcus pneumoniae D39	56.774	95.679	0.543
ruvB	Synechocystis sp. PCC 6803	50.779	99.074	0.503
ruvB	Helicobacter pylori 26695	50.637	96.914	0.491