Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PUN50_RS02915 Genome accession   NZ_CP117988
Coordinates   600757..601224 (-) Length   155 a.a.
NCBI ID   WP_274290834.1    Uniprot ID   -
Organism   Vibrio campbellii strain 20220413_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 595757..606224
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN50_RS02895 (PUN50_02895) coaE 596257..596871 (-) 615 WP_045369833.1 dephospho-CoA kinase -
  PUN50_RS02900 (PUN50_02900) pilD 596872..597741 (-) 870 WP_274290832.1 prepilin peptidase Machinery gene
  PUN50_RS02905 (PUN50_02905) pilC 597814..599037 (-) 1224 WP_045451199.1 type II secretion system F family protein Machinery gene
  PUN50_RS02910 (PUN50_02910) pilB 599072..600757 (-) 1686 WP_274290833.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PUN50_RS02915 (PUN50_02915) pilA 600757..601224 (-) 468 WP_274290834.1 pilin Machinery gene
  PUN50_RS02920 (PUN50_02920) nadC 601490..602377 (-) 888 WP_012128875.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PUN50_RS02925 (PUN50_02925) ampD 602470..603021 (+) 552 WP_038891990.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PUN50_RS02930 (PUN50_02930) pdhR 603426..604193 (+) 768 WP_005428674.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 15812.94 Da        Isoelectric Point: 5.0330

>NTDB_id=720177 PUN50_RS02915 WP_274290834.1 600757..601224(-) (pilA) [Vibrio campbellii strain 20220413_1]
MKTNKQKKQQGFTLIELMIVVAIIGILAAFAVPAYQNYTKSTHASEMASATAAFKTAVGICLLDGQADCTDGTGGVPAEQ
AFTKDATDSFTVSSTVEQKTIGTATGAILASVSGAKGALPSDAQVVLTPTISTSGVTWAITCSGTGTTGDWCPER

Nucleotide


Download         Length: 468 bp        

>NTDB_id=720177 PUN50_RS02915 WP_274290834.1 600757..601224(-) (pilA) [Vibrio campbellii strain 20220413_1]
ATGAAAACGAATAAACAGAAGAAGCAGCAAGGTTTTACGCTGATTGAATTGATGATTGTGGTAGCGATTATTGGTATTTT
GGCAGCATTTGCTGTGCCAGCGTATCAAAATTATACAAAAAGCACTCATGCAAGTGAAATGGCAAGTGCAACAGCCGCTT
TCAAAACTGCTGTTGGTATTTGTCTACTGGATGGCCAAGCCGATTGTACAGATGGAACTGGAGGCGTTCCCGCAGAACAA
GCGTTCACTAAAGATGCTACTGACAGTTTCACCGTATCTTCAACAGTTGAACAGAAAACGATTGGAACAGCGACTGGTGC
AATCTTAGCTTCAGTTAGTGGTGCAAAAGGAGCTTTACCTTCCGATGCACAAGTAGTGTTGACTCCAACAATCTCAACTT
CAGGTGTAACTTGGGCGATTACATGTTCAGGCACAGGAACAACTGGTGACTGGTGTCCTGAACGATAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

42.073

100

0.445
  pilA2 Legionella pneumophila str. Paris

41.304

89.032

0.368
  pilA Haemophilus influenzae 86-028NP

39.31

93.548

0.368
  pilA2 Legionella pneumophila strain ERS1305867

40.58

89.032

0.361