NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	NW339_RS02205	Genome accession	NZ_CP102947
Coordinates	451951..453075 (+)	Length	374 a.a.
NCBI ID	WP_000228517.1	Uniprot ID	E2QFE2
Organism	Escherichia coli strain SCAID WND1-2022 (119)
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 446951..458075

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NW339_RS02180 (NW339_02180)	mscL	447068..447481 (-)	414	WP_000022450.1	large-conductance mechanosensitive channel protein MscL	-
NW339_RS02185 (NW339_02185)	trkA	447611..448987 (-)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
NW339_RS02190 (NW339_02190)	rsmB	449009..450298 (-)	1290	WP_000744765.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
NW339_RS02195 (NW339_02195)	fmt	450350..451297 (-)	948	WP_000004421.1	methionyl-tRNA formyltransferase	-
NW339_RS02200 (NW339_02200)	def	451312..451821 (-)	510	WP_000114984.1	peptide deformylase	-
NW339_RS02205 (NW339_02205)	dprA	451951..453075 (+)	1125	WP_000228517.1	DNA-protecting protein DprA	Machinery gene
NW339_RS02210 (NW339_02210)	smg	453047..453520 (+)	474	WP_000460673.1	DUF494 family protein Smg	-
NW339_RS02215 (NW339_02215)	yrdD	453549..454091 (+)	543	WP_001296461.1	type I DNA topoisomerase	-
NW339_RS02220 (NW339_02220)	tsaC	454096..454668 (+)	573	WP_001296460.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
NW339_RS02225 (NW339_02225)	aroE	454673..455491 (+)	819	WP_000451255.1	shikimate dehydrogenase	-
NW339_RS02230 (NW339_02230)	yrdB	455488..455745 (+)	258	WP_001070565.1	DUF1488 domain-containing protein	-
NW339_RS02235 (NW339_02235)	yrdA	455721..456275 (-)	555	WP_000005712.1	gamma carbonic anhydrase family protein	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40910.86 Da Isoelectric Point: 6.0395

                                >NTDB_id=720137 NW339_RS02205 WP_000228517.1 451951..453075(+) (dprA) [Escherichia coli strain SCAID WND1-2022 (119)]

MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=720137 NW339_RS02205 WP_000228517.1 451951..453075(+) (dprA) [Escherichia coli strain SCAID WND1-2022 (119)]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT

GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT

TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGTCTAAGTAAGGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	E2QFE2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.78	98.663	0.481
dprA	Vibrio cholerae strain A1552	49.355	82.888	0.409
dprA	Glaesserella parasuis strain SC1401	44.807	90.107	0.404
dprA	Haemophilus influenzae Rd KW20	44.214	90.107	0.398
dprA	Legionella pneumophila strain ERS1305867	44.242	88.235	0.39

Detailed information NTG0720137