Detailed information NTG0720101

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NW341_RS02000
• Genome accession: NZ_CP102946
• Coordinates: 430802..431950 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain SCAID WND1-2022 (148)
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 425802..436950

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NW341_RS01975 (NW341_01975)	-	426953..427249 (-)	297	WP_033877412.1	DUF167 domain-containing protein	-
NW341_RS01980 (NW341_01980)	-	427246..427839 (-)	594	WP_003084544.1	YggT family protein	-
NW341_RS01985 (NW341_01985)	proC	427850..428671 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
NW341_RS01990 (NW341_01990)	-	428683..429375 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
NW341_RS01995 (NW341_01995)	pilT	429590..430624 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
NW341_RS02000 (NW341_02000)	pilU	430802..431950 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
NW341_RS02005 (NW341_02005)	czcD	431958..432857 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
NW341_RS02010 (NW341_02010)	-	433011..433415 (+)	405	WP_003084560.1	NINE protein	-
NW341_RS02015 (NW341_02015)	-	433658..435031 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
NW341_RS02020 (NW341_02020)	-	435028..436212 (+)	1185	WP_033877411.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=720101 NW341_RS02000 WP_003100959.1 430802..431950(+) (pilU) [Pseudomonas aeruginosa strain SCAID WND1-2022 (148)]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=720101 NW341_RS02000 WP_003100959.1 430802..431950(+) (pilU) [Pseudomonas aeruginosa strain SCAID WND1-2022 (148)]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382