Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   MHI66_RS18975 Genome accession   NZ_CP102913
Coordinates   3960475..3960921 (-) Length   148 a.a.
NCBI ID   WP_003361810.1    Uniprot ID   A0A846I524
Organism   Clostridium botulinum strain FE9508BRB     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context

Location: 3955475..3965921
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI66_RS18950 (MHI66_003775) rplI 3956304..3956747 (-) 444 WP_003359455.1 50S ribosomal protein L9 -
  MHI66_RS18955 (MHI66_003776) - 3956747..3958732 (-) 1986 WP_003361808.1 DHH family phosphoesterase -
  MHI66_RS18960 (MHI66_003777) - 3958733..3959737 (-) 1005 WP_003361809.1 YybS family protein -
  MHI66_RS18965 (MHI66_003778) - 3959749..3960066 (-) 318 WP_003359330.1 MazG-like family protein -
  MHI66_RS18970 (MHI66_003779) rpsR 3960214..3960456 (-) 243 WP_003359407.1 30S ribosomal protein S18 -
  MHI66_RS18975 (MHI66_003780) ssbA 3960475..3960921 (-) 447 WP_003361810.1 single-stranded DNA-binding protein Machinery gene
  MHI66_RS18980 (MHI66_003781) rpsF 3960937..3961221 (-) 285 WP_003361811.1 30S ribosomal protein S6 -
  MHI66_RS18985 (MHI66_003782) - 3961327..3962475 (-) 1149 WP_003361812.1 double-cubane-cluster-containing anaerobic reductase -
  MHI66_RS18990 (MHI66_003783) - 3962741..3962938 (-) 198 WP_003361813.1 DUF951 domain-containing protein -
  MHI66_RS18995 (MHI66_003784) - 3962954..3963823 (-) 870 WP_003361814.1 mechanosensitive ion channel family protein -
  MHI66_RS19000 (MHI66_003785) - 3963830..3964075 (-) 246 WP_003361815.1 DUF3343 domain-containing protein -
  MHI66_RS19005 (MHI66_003786) - 3964266..3965420 (+) 1155 WP_003361816.1 aminotransferase class V-fold PLP-dependent enzyme -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 16424.29 Da        Isoelectric Point: 4.9816

>NTDB_id=719689 MHI66_RS18975 WP_003361810.1 3960475..3960921(-) (ssbA) [Clostridium botulinum strain FE9508BRB]
MNKVVLIGRLTKDPELKFTPGNGTAVATFTLAVDRRFSKDGQREADFIPIVVWGKQAESTANYMSKGKLMGVSGRIQTRS
YEAKDGTRRYVTEVIAEEVKFLEWGNKPTTDSTYNNGSEPSYQSGQGFRNGNSFDDDIIPVDDGDIPF

Nucleotide


Download         Length: 447 bp        

>NTDB_id=719689 MHI66_RS18975 WP_003361810.1 3960475..3960921(-) (ssbA) [Clostridium botulinum strain FE9508BRB]
ATGAATAAAGTTGTTTTGATAGGTAGATTAACCAAAGATCCTGAGTTAAAATTTACTCCAGGAAACGGGACAGCAGTTGC
CACCTTTACTTTGGCTGTAGATAGAAGATTTTCAAAAGATGGTCAGAGAGAAGCAGATTTCATCCCTATAGTAGTATGGG
GAAAACAAGCAGAATCTACAGCTAACTACATGAGTAAAGGAAAACTTATGGGGGTTAGTGGTAGAATTCAAACAAGAAGC
TATGAAGCTAAAGATGGTACAAGAAGATATGTAACAGAAGTCATAGCTGAAGAAGTTAAATTTCTTGAGTGGGGCAATAA
GCCTACAACGGATTCAACATATAATAATGGGTCAGAACCAAGTTATCAATCAGGCCAAGGCTTTAGAAATGGAAACAGTT
TTGACGACGATATAATTCCAGTGGATGATGGAGACATCCCATTTTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(1-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A846I524

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

48.837

100

0.568
  ssb Latilactobacillus sakei subsp. sakei 23K

43.919

100

0.439
  ssbB Bacillus subtilis subsp. subtilis str. 168

55.34

69.595

0.385
  ssbB Streptococcus sobrinus strain NIDR 6715-7

53.398

69.595

0.372