Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PUO95_RS03450 Genome accession   NZ_CP117823
Coordinates   816449..817507 (+) Length   352 a.a.
NCBI ID   WP_004089911.1    Uniprot ID   B2I8B6
Organism   Xylella fastidiosa subsp. fastidiosa strain ICMP 8742     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 811449..822507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUO95_RS03440 (PUO95_03440) - 812511..813476 (+) 966 WP_004089914.1 aldo/keto reductase -
  PUO95_RS03445 (PUO95_03445) - 813780..816209 (-) 2430 WP_012382725.1 penicillin-binding protein 1A -
  PUO95_RS03450 (PUO95_03450) pilM 816449..817507 (+) 1059 WP_004089911.1 type IV pilus biogenesis protein PilM Machinery gene
  PUO95_RS03455 (PUO95_03455) - 817507..818067 (+) 561 Protein_662 PilN domain-containing protein -
  PUO95_RS03460 (PUO95_03460) - 818169..818837 (+) 669 WP_004089909.1 type 4a pilus biogenesis protein PilO -
  PUO95_RS03465 (PUO95_03465) - 818834..819364 (+) 531 WP_011098214.1 pilus assembly protein PilP -
  PUO95_RS03470 (PUO95_03470) comQ/pilQ 819364..821274 (+) 1911 WP_011098213.1 type IV pilus secretin PilQ Machinery gene

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37539.19 Da        Isoelectric Point: 4.6541

>NTDB_id=719495 PUO95_RS03450 WP_004089911.1 816449..817507(+) (pilM) [Xylella fastidiosa subsp. fastidiosa strain ICMP 8742]
MGLFSKKQSVLVGVDISSTAVKLLQLSRSGNRFKVEHYAVEPLPLNAVAEKGIVEVEQVGEAIRRAVSRSGTKAKFAAAA
VAGSAVITKLIPMPAGLDEQDLEAQIEIEATNYIPYPIEEVSLDFEVLGPVPNNTEMVQVLLAASRSENVELRQSALELG
GLTAKVIDVEALAVENAFSLIAQELSVGSNALVALIDIGATMSTLNVLHSGRSLYAREQLFGGKQLTDEVMHRYGMTYEE
AGQAKRQGGLPQSYEVEVLGPFKDSVIQQISRLLQFFYAGSEYNRVDCIVLAGGCAVIAGLPAMVEERLGVVTVVANPLA
QMTLGAKVQAHTLAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=719495 PUO95_RS03450 WP_004089911.1 816449..817507(+) (pilM) [Xylella fastidiosa subsp. fastidiosa strain ICMP 8742]
GTGGGGCTTTTTTCAAAAAAACAGTCGGTATTGGTTGGTGTCGACATTAGTTCAACTGCAGTGAAGCTCTTACAGCTTTC
CCGCAGTGGTAATCGCTTCAAGGTGGAACACTATGCTGTGGAGCCGCTACCTCTGAATGCGGTCGCTGAGAAGGGCATCG
TTGAAGTCGAGCAGGTTGGTGAAGCAATCCGTCGTGCGGTAAGTCGTTCTGGAACTAAGGCCAAATTTGCCGCTGCCGCG
GTTGCCGGTTCGGCAGTGATCACCAAATTGATACCGATGCCTGCTGGTCTGGACGAGCAGGATTTGGAAGCTCAGATAGA
AATCGAGGCTACGAATTACATCCCGTACCCTATTGAAGAGGTCAGCCTAGATTTCGAGGTGCTTGGTCCGGTTCCCAACA
ACACGGAGATGGTCCAAGTTTTGCTGGCTGCGTCTCGTTCGGAGAATGTCGAATTGCGCCAATCTGCGTTGGAGCTGGGG
GGGTTGACTGCCAAGGTTATTGACGTGGAAGCTTTAGCGGTTGAGAACGCTTTCTCTCTTATTGCCCAAGAACTGTCGGT
TGGGAGTAATGCATTGGTTGCATTGATCGACATCGGTGCAACGATGTCGACGCTTAATGTGCTGCATAGTGGTCGCAGTT
TGTACGCGCGCGAGCAGCTATTTGGTGGTAAGCAGCTTACTGACGAAGTAATGCATCGCTATGGGATGACTTACGAGGAG
GCTGGTCAGGCCAAGCGTCAAGGAGGTTTGCCGCAAAGCTATGAGGTTGAAGTGCTGGGACCATTTAAGGACTCTGTGAT
CCAGCAGATCAGCCGTTTATTACAGTTTTTCTATGCTGGCAGTGAGTACAACCGAGTTGATTGCATCGTGTTGGCGGGAG
GCTGTGCTGTGATTGCGGGGTTACCGGCTATGGTCGAAGAACGGTTGGGTGTCGTTACCGTTGTGGCTAATCCGCTTGCG
CAGATGACTTTAGGGGCGAAAGTGCAAGCACACACGCTTGCTCAAGATGCACCTGCTTTAATGATTGCTACTGGTCTGGC
TTTGAGGAGTTTTGACTGA

Domains

Predicted by InterProScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B2I8B6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

48.864

100

0.489
  comM Acinetobacter nosocomialis M2

48.864

100

0.489
  comM/pilM Acinetobacter baylyi ADP1

48.011

100

0.48
  pilM Legionella pneumophila strain ERS1305867

45.609

100

0.457