Detailed information NTG0719214

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PTC86_RS16985
• Genome accession: NZ_CP117764
• Coordinates: 3578752..3579870 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain 2022CK-00784
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3573752..3584870

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PTC86_RS16955 (PTC86_16955)	-	3574581..3575429 (+)	849	WP_000336548.1	class II glutamine amidotransferase	-
PTC86_RS16960 (PTC86_16960)	-	3575682..3576137 (+)	456	WP_000782976.1	bacteriohemerythrin	-
PTC86_RS16965 (PTC86_16965)	-	3576290..3577357 (+)	1068	WP_002019572.1	hypothetical protein	-
PTC86_RS16970 (PTC86_16970)	-	3577357..3577680 (+)	324	WP_000442587.1	RnfH family protein	-
PTC86_RS16975 (PTC86_16975)	-	3577735..3578133 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
PTC86_RS16980 (PTC86_16980)	fur	3578245..3578682 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
PTC86_RS16985 (PTC86_16985)	pilU	3578752..3579870 (-)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PTC86_RS16990 (PTC86_16990)	pilT	3579898..3580935 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
PTC86_RS16995 (PTC86_16995)	-	3581062..3581754 (+)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=719214 PTC86_RS16985 WP_000347039.1 3578752..3579870(-) (pilU) [Acinetobacter baumannii strain 2022CK-00784]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=719214 PTC86_RS16985 WP_000347039.1 3578752..3579870(-) (pilU) [Acinetobacter baumannii strain 2022CK-00784]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395