Detailed information NTG0719192

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NV385_RS14850
• Genome accession: NZ_CP102831
• Coordinates: 3037046..3038164 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain AB3927
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3032046..3043164

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NV385_RS14840 (NV385_14840)	-	3035162..3035854 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
NV385_RS14845 (NV385_14845)	pilT	3035981..3037018 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
NV385_RS14850 (NV385_14850)	pilU	3037046..3038164 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NV385_RS14855 (NV385_14855)	fur	3038234..3038671 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
NV385_RS14860 (NV385_14860)	-	3038783..3039181 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
NV385_RS14865 (NV385_14865)	-	3039236..3039559 (-)	324	WP_000442587.1	RnfH family protein	-
NV385_RS14870 (NV385_14870)	-	3039559..3040626 (-)	1068	WP_002019572.1	hypothetical protein	-
NV385_RS14875 (NV385_14875)	-	3040779..3041234 (-)	456	WP_000782976.1	bacteriohemerythrin	-
NV385_RS14880 (NV385_14880)	-	3041487..3042335 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=719192 NV385_RS14850 WP_000347039.1 3037046..3038164(+) (pilU) [Acinetobacter baumannii strain AB3927]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=719192 NV385_RS14850 WP_000347039.1 3037046..3038164(+) (pilU) [Acinetobacter baumannii strain AB3927]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395