Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   LOY40_RS02275 Genome accession   NZ_CP102179
Coordinates   508279..509343 (+) Length   354 a.a.
NCBI ID   WP_030140991.1    Uniprot ID   -
Organism   Pseudomonas alvandae strain B21-020     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 503279..514343
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY40_RS02265 (LOY40_02265) - 504057..505325 (+) 1269 WP_030140993.1 malic enzyme-like NAD(P)-binding protein -
  LOY40_RS02270 (LOY40_02270) - 505626..508070 (-) 2445 WP_187293336.1 penicillin-binding protein 1A -
  LOY40_RS02275 (LOY40_02275) comM 508279..509343 (+) 1065 WP_030140991.1 pilus assembly protein PilM Machinery gene
  LOY40_RS02280 (LOY40_02280) - 509343..509909 (+) 567 WP_030140990.1 PilN domain-containing protein -
  LOY40_RS02285 (LOY40_02285) pilO 509906..510529 (+) 624 WP_030140989.1 type 4a pilus biogenesis protein PilO -
  LOY40_RS02290 (LOY40_02290) - 510526..511053 (+) 528 WP_030140988.1 pilus assembly protein PilP -
  LOY40_RS02295 (LOY40_02295) pilQ 511067..513139 (+) 2073 WP_217861073.1 type IV pilus secretin PilQ Machinery gene
  LOY40_RS02300 (LOY40_02300) aroK 513144..513662 (+) 519 WP_033864550.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38127.67 Da        Isoelectric Point: 4.8313

>NTDB_id=715289 LOY40_RS02275 WP_030140991.1 508279..509343(+) (comM) [Pseudomonas alvandae strain B21-020]
MLRLFNKKAHGLLGIDISSTSVKLLELSRQGDRYRVESYAVEPLPANAVIEKNIAELEGVGMALSRVLDKARTPLRNVAV
AVAGSAVITKTIEMDAGLSEDDMENQLKIEADQYIPYPLDEVAIDFEVVGASPRSTERVEVLLAACRKENVEVREAALGL
AGLTARVVDVEAYALERAFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTP
EQAGLAKKQGGLPDDYLGEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGTASVAGLDRLIEQRLGTPTQVANP
FTNMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=715289 LOY40_RS02275 WP_030140991.1 508279..509343(+) (comM) [Pseudomonas alvandae strain B21-020]
GTGCTGCGACTCTTCAATAAAAAAGCCCACGGGCTTTTGGGCATCGACATCAGTTCAACATCGGTCAAGCTGCTCGAGTT
GAGCCGCCAGGGCGACCGATACCGCGTCGAGTCCTATGCGGTCGAACCGCTGCCGGCCAACGCAGTGATCGAGAAGAATA
TCGCCGAGCTCGAAGGGGTGGGCATGGCGTTGTCGCGGGTGCTCGACAAGGCCCGGACGCCCTTGCGCAACGTGGCGGTC
GCGGTTGCGGGGTCGGCGGTCATTACCAAGACCATCGAGATGGACGCCGGGTTGTCGGAAGATGACATGGAAAACCAGCT
CAAGATCGAGGCCGACCAATACATTCCTTATCCGCTGGATGAGGTGGCGATCGATTTCGAGGTGGTGGGTGCTTCTCCCC
GCAGCACCGAACGGGTCGAAGTGCTGCTGGCGGCTTGTCGAAAGGAGAACGTCGAGGTCCGCGAGGCGGCGCTGGGGCTC
GCCGGGCTGACGGCTCGCGTGGTCGATGTCGAAGCCTACGCGCTGGAGCGAGCCTTCGGTCTGCTCGCCACGCAACTTGC
CGCGTCCCAGGAGCGACTGACCGTGGCGGTCGTCGACATCGGCGCCACCATGACCACGCTCAGCGTGTTGCACAACGGTC
GCATCATTTATACCCGTGAGCAGTTGTTCGGGGGGCGTCAGCTTACCGAGGAAATCCAGCGTCGCTATGGCCTGACGCCA
GAGCAGGCCGGCCTGGCGAAAAAGCAGGGCGGTTTGCCCGACGATTATCTCGGCGAGGTGCTTCAACCGTTTCGCGAAGC
GCTGGTGCAGCAGGTCTCGCGTTCGTTGCAGTTTTTCTTCGCCTCGGGTCAGTACAGCGCGGTGGATCACATCCTGCTGG
CGGGAGGCACTGCGTCAGTCGCCGGCCTGGACCGCCTGATCGAGCAGCGCCTGGGCACGCCAACCCAGGTCGCCAACCCG
TTTACCAATATGGCCCTGAGTAGCAAGGTCAACGCCGGCGCCCTGGCCAGCGATGCGCCGGCGCTGATGATCGCCTGCGG
GTTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  pilM Acinetobacter baumannii D1279779

55.65

100

0.557
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

46.893

100

0.469