Detailed information NTG0715247

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: LOY71_RS22370
• Genome accession: NZ_CP102176
• Coordinates: 5169729..5170598 (+)
• Length: 289 a.a.
• NCBI ID: WP_047698305.1
• Uniprot ID: A0AAX2D8P6
• Organism: Pseudomonas mediterranea strain B21-060
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5164729..5175598

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LOY71_RS22345 (LOY71_22350)	-	5164914..5165210 (+)	297	WP_047698296.1	DUF2845 domain-containing protein	-
LOY71_RS22350 (LOY71_22355)	-	5165510..5165863 (-)	354	WP_047698298.1	BON domain-containing protein	-
LOY71_RS22355 (LOY71_22360)	-	5166161..5166583 (-)	423	WP_047698300.1	pilin	-
LOY71_RS22360 (LOY71_22365)	pilB	5166807..5168507 (+)	1701	WP_047698302.1	type IV-A pilus assembly ATPase PilB	Machinery gene
LOY71_RS22365 (LOY71_22370)	pilC	5168510..5169727 (+)	1218	WP_047698304.1	type II secretion system F family protein	Machinery gene
LOY71_RS22370 (LOY71_22375)	pilD	5169729..5170598 (+)	870	WP_047698305.1	A24 family peptidase	Machinery gene
LOY71_RS22375 (LOY71_22380)	coaE	5170609..5171232 (+)	624	WP_047698307.1	dephospho-CoA kinase	-
LOY71_RS22380 (LOY71_22385)	yacG	5171229..5171435 (+)	207	WP_047698308.1	DNA gyrase inhibitor YacG	-
LOY71_RS22385 (LOY71_22390)	-	5171432..5171647 (-)	216	WP_047698310.1	hypothetical protein	-
LOY71_RS22390 (LOY71_22395)	-	5171711..5172397 (-)	687	WP_047698311.1	energy-coupling factor ABC transporter permease	-
LOY71_RS22395 (LOY71_22400)	-	5172617..5173243 (+)	627	WP_003185538.1	DUF1780 domain-containing protein	-
LOY71_RS22400 (LOY71_22405)	-	5173345..5173518 (+)	174	WP_003185543.1	DUF3094 domain-containing protein	-
LOY71_RS22405 (LOY71_22410)	-	5173600..5174898 (+)	1299	WP_047698313.1	NAD(P)/FAD-dependent oxidoreductase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32000.13 Da
• Isoelectric Point: 8.0659

>NTDB_id=715247 LOY71_RS22370 WP_047698305.1 5169729..5170598(+) (pilD) [Pseudomonas mediterranea strain B21-060]
MPLSEFFVLYPMAFVLTALLLGLIVGSFLNVLVWRLPKMLTREWRLQAHDLLGLPAETPGPVYNLMLPHSQCPHCGHRIR
AWENIPLFSYLALHGRCSSCATPIGRRYPLTELACGALSAFVAWHFGFGWQAALVMVLSWGLLGMSLIDAEHQLLPDTLV
LPLLWLGLIVNSFGLFVSLNQAMWGAVAGYLTLWSVFWIFKLITGKEGMGYGDFKLLAMLGAWGGWQILPLTLLLSSLVG
AVVGVVMLRMRNAPASTQIPFGPYLAIAGWIALLWGGQITDFYWHSVGF

Nucleotide

• Length: 870 bp

>NTDB_id=715247 LOY71_RS22370 WP_047698305.1 5169729..5170598(+) (pilD) [Pseudomonas mediterranea strain B21-060]
ATGCCCCTGAGCGAATTTTTCGTGCTTTATCCCATGGCCTTCGTGCTGACAGCGCTTTTGCTCGGGCTGATCGTCGGCAG
CTTCCTCAATGTCCTGGTGTGGCGCCTGCCCAAGATGCTCACCCGGGAATGGCGACTACAGGCCCACGACCTGCTGGGCC
TGCCTGCCGAAACGCCCGGCCCGGTCTACAACCTGATGCTGCCCCATTCCCAATGCCCTCACTGCGGCCACCGTATCCGG
GCCTGGGAAAATATCCCGTTGTTCAGCTACCTCGCACTGCATGGCCGCTGTTCCAGTTGCGCCACCCCCATCGGGCGCCG
TTACCCTCTTACCGAGCTGGCCTGCGGCGCACTGTCGGCATTCGTCGCCTGGCATTTCGGCTTCGGTTGGCAGGCCGCGC
TGGTGATGGTCCTGAGCTGGGGCCTGCTGGGCATGAGCCTGATCGATGCCGAGCATCAACTGCTGCCCGATACACTGGTG
CTGCCGTTATTGTGGCTGGGGCTGATCGTCAACAGCTTCGGGCTGTTCGTCTCGCTGAACCAGGCGATGTGGGGCGCAGT
GGCCGGCTACCTGACGTTGTGGTCGGTGTTCTGGATCTTCAAGTTGATCACCGGCAAGGAAGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCGATGCTCGGGGCCTGGGGCGGCTGGCAGATCCTGCCACTGACGCTGCTGCTGTCGTCGCTGGTGGGC
GCCGTGGTCGGGGTCGTCATGTTGCGCATGCGCAATGCTCCGGCGTCGACACAGATCCCCTTCGGCCCCTATTTGGCCAT
TGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGACTTCTATTGGCACTCTGTCGGTTTCTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.113	98.27	0.512
pilD	Vibrio campbellii strain DS40M4	49.474	98.616	0.488
pilD	Neisseria gonorrhoeae MS11	46.341	99.308	0.46
pilD	Acinetobacter baumannii D1279779	46.263	97.232	0.45
pilD	Acinetobacter nosocomialis M2	46.263	97.232	0.45