Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   NL422_RS04525 Genome accession   NZ_CP116931
Coordinates   811060..812553 (+) Length   497 a.a.
NCBI ID   WP_072617370.1    Uniprot ID   -
Organism   Escherichia coli strain MLI106K1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 806060..817553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NL422_RS04505 (NL422_004505) pilD 806708..807517 (+) 810 WP_069903752.1 prepilin peptidase PppA Machinery gene
  NL422_RS04510 (NL422_004510) gspS2 807583..807993 (+) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  NL422_RS04515 (NL422_004515) gspC 808011..808970 (+) 960 WP_072721285.1 type II secretion system protein GspC -
  NL422_RS04520 (NL422_004520) gspD 809000..811060 (+) 2061 WP_000498829.1 type II secretion system secretin GspD -
  NL422_RS04525 (NL422_004525) pilB/pilB1 811060..812553 (+) 1494 WP_072617370.1 type II secretion system ATPase GspE Machinery gene
  NL422_RS04530 (NL422_004530) gspF 812553..813776 (+) 1224 WP_072617369.1 type II secretion system inner membrane protein GspF -
  NL422_RS04535 (NL422_004535) gspG 813793..814248 (+) 456 WP_001087289.1 type II secretion system major pseudopilin GspG -
  NL422_RS04540 (NL422_004540) gspH 814252..814815 (+) 564 WP_021513238.1 type II secretion system minor pseudopilin GspH -
  NL422_RS04545 (NL422_004545) gspI 814812..815183 (+) 372 WP_072617368.1 type II secretion system minor pseudopilin GspI -
  NL422_RS04550 (NL422_004550) gspJ 815180..815785 (+) 606 WP_021513237.1 type II secretion system minor pseudopilin GspJ -
  NL422_RS04555 (NL422_004555) gspK 815782..816759 (+) 978 WP_049067520.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55261.37 Da        Isoelectric Point: 6.1287

>NTDB_id=714520 NL422_RS04525 WP_072617370.1 811060..812553(+) (pilB/pilB1) [Escherichia coli strain MLI106K1]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPSIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQAAPA
TDEEKRLLRITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=714520 NL422_RS04525 WP_072617370.1 811060..812553(+) (pilB/pilB1) [Escherichia coli strain MLI106K1]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTTTGGCAGCGTGACTCTTCC
GAGGCTCGCCAGCTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCAGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGAGCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATTAAGGTGATGGCACGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCATCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCACGAACTGACC
GCGCAGTTGCGCCAGCTACTGCACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGTAACATTCTCACGGTTGAAGATCCCATCGAATACATGATTG
AAGGGATCGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTTGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTTCAGGCGTCGCTCACTGGGCACCT
GGTCCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATTACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGTGTGATGGCGCAGCGACTGGTCCGCACGTTGTGCCCCGACTGCCGTCAGGCCGCGCCTGCT
ACTGACGAAGAAAAACGCCTGCTGAGGATTACCGACGCGCGTACTGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGTTGATCGTGGTGGACGCCACATTGCGTGATCTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGACAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.926

100

0.435
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.059

85.513

0.402
  pilB Vibrio parahaemolyticus RIMD 2210633

47.99

80.08

0.384
  pilB Acinetobacter baumannii D1279779

46.287

81.288

0.376
  pilB Vibrio campbellii strain DS40M4

46.97

79.678

0.374
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilB Legionella pneumophila strain ERS1305867

42.459

86.72

0.368
  pilF Thermus thermophilus HB27

44.75

80.483

0.36