Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   FFJ14_RS13905 Genome accession   NZ_AP024126
Coordinates   2839984..2840994 (-) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain NS-NP030     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2834984..2845994
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FFJ14_RS13885 (BvCmsNSNP030_2738) mepM 2835951..2837273 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  FFJ14_RS13890 (BvCmsNSNP030_2739) znuA 2837289..2838221 (-) 933 WP_001342995.1 zinc ABC transporter substrate-binding protein ZnuA -
  FFJ14_RS13895 (BvCmsNSNP030_2740) znuC 2838300..2839055 (+) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  FFJ14_RS13900 (BvCmsNSNP030_2741) znuB 2839052..2839837 (+) 786 WP_000571465.1 zinc ABC transporter permease subunit ZnuB -
  FFJ14_RS13905 (BvCmsNSNP030_2742) ruvB 2839984..2840994 (-) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  FFJ14_RS13910 (BvCmsNSNP030_2743) ruvA 2841003..2841614 (-) 612 WP_000580328.1 Holliday junction branch migration protein RuvA -
  FFJ14_RS13915 yobI 2841753..2841818 (-) 66 WP_001386853.1 stress response small protein YobI -
  FFJ14_RS13920 (BvCmsNSNP030_2744) yebB 2841889..2842491 (+) 603 WP_001024917.1 YebB family permuted papain-like enzyme -
  FFJ14_RS13925 (BvCmsNSNP030_2745) ruvC 2842493..2843014 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  FFJ14_RS13930 (BvCmsNSNP030_2746) yebC 2843049..2843789 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  FFJ14_RS13935 (BvCmsNSNP030_2747) nudB 2843818..2844270 (-) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=71437 FFJ14_RS13905 WP_000568519.1 2839984..2840994(-) (ruvB) [Escherichia coli strain NS-NP030]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=71437 FFJ14_RS13905 WP_000568519.1 2839984..2840994(-) (ruvB) [Escherichia coli strain NS-NP030]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTCGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGTTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGAGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment