Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   DAECLI1_RS02185 Genome accession   NZ_AP018784
Coordinates   449548..450672 (+) Length   374 a.a.
NCBI ID   WP_000228517.1    Uniprot ID   E2QFE2
Organism   Escherichia coli strain SK1144     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 444548..455672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DAECLI1_RS02160 (DAECLI1_04270) mscL 444665..445078 (-) 414 WP_000022450.1 large-conductance mechanosensitive channel protein MscL -
  DAECLI1_RS02165 (DAECLI1_04280) trkA 445208..446584 (-) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  DAECLI1_RS02170 (DAECLI1_04290) rsmB 446606..447895 (-) 1290 WP_000744765.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  DAECLI1_RS02175 (DAECLI1_04300) fmt 447947..448894 (-) 948 WP_000004421.1 methionyl-tRNA formyltransferase -
  DAECLI1_RS02180 (DAECLI1_04310) def 448909..449418 (-) 510 WP_000114984.1 peptide deformylase -
  DAECLI1_RS02185 (DAECLI1_04320) dprA 449548..450672 (+) 1125 WP_000228517.1 DNA-protecting protein DprA Machinery gene
  DAECLI1_RS02190 (DAECLI1_04330) smg 450644..451117 (+) 474 WP_000460673.1 DUF494 family protein Smg -
  DAECLI1_RS02195 yrdD 451146..451688 (+) 543 WP_001296461.1 type I DNA topoisomerase -
  DAECLI1_RS02200 (DAECLI1_04350) tsaC 451693..452265 (+) 573 WP_001296460.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  DAECLI1_RS02205 (DAECLI1_04360) aroE 452270..453088 (+) 819 WP_000451255.1 shikimate dehydrogenase -
  DAECLI1_RS02210 (DAECLI1_04370) yrdB 453085..453342 (+) 258 WP_001070565.1 DUF1488 domain-containing protein -
  DAECLI1_RS02215 (DAECLI1_04380) yrdA 453318..453872 (-) 555 WP_000005712.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40910.86 Da        Isoelectric Point: 6.0395

>NTDB_id=71408 DAECLI1_RS02185 WP_000228517.1 449548..450672(+) (dprA) [Escherichia coli strain SK1144]
MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=71408 DAECLI1_RS02185 WP_000228517.1 449548..450672(+) (dprA) [Escherichia coli strain SK1144]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT
TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAGGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E2QFE2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.78

98.663

0.481
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Glaesserella parasuis strain SC1401

44.807

90.107

0.404
  dprA Haemophilus influenzae Rd KW20

44.214

90.107

0.398
  dprA Legionella pneumophila strain ERS1305867

44.242

88.235

0.39

Multiple sequence alignment