Detailed information NTG0712743

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NP446_RS02065
• Genome accession: NZ_CP101912
• Coordinates: 447449..448597 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain ATCC 27853
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 442449..453597

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NP446_RS02040 (NP446_02040)	-	443600..443896 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
NP446_RS02045 (NP446_02045)	-	443893..444486 (-)	594	WP_003084544.1	YggT family protein	-
NP446_RS02050 (NP446_02050)	proC	444497..445318 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
NP446_RS02055 (NP446_02055)	-	445330..446022 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
NP446_RS02060 (NP446_02060)	pilT	446237..447271 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
NP446_RS02065 (NP446_02065)	pilU	447449..448597 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
NP446_RS02070 (NP446_02070)	czcD	448605..449504 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
NP446_RS02075 (NP446_02075)	-	449659..450063 (+)	405	WP_003084560.1	NINE protein	-
NP446_RS02080 (NP446_02080)	-	450306..451679 (+)	1374	WP_003459266.1	pyridoxal-phosphate dependent enzyme	-
NP446_RS02085 (NP446_02085)	-	451676..452860 (+)	1185	WP_016852373.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=712743 NP446_RS02065 WP_003100959.1 447449..448597(+) (pilU) [Pseudomonas aeruginosa strain ATCC 27853]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=712743 NP446_RS02065 WP_003100959.1 447449..448597(+) (pilU) [Pseudomonas aeruginosa strain ATCC 27853]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382