Detailed information NTG0712709

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NP444_RS01995
• Genome accession: NZ_CP101911
• Coordinates: 430664..431812 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain NWRC-1223
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 425664..436812

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NP444_RS01970 (NP444_01970)	-	426815..427111 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
NP444_RS01975 (NP444_01975)	-	427108..427701 (-)	594	WP_003084544.1	YggT family protein	-
NP444_RS01980 (NP444_01980)	proC	427712..428533 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
NP444_RS01985 (NP444_01985)	-	428545..429237 (-)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
NP444_RS01990 (NP444_01990)	pilT	429452..430486 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
NP444_RS01995 (NP444_01995)	pilU	430664..431812 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
NP444_RS02000 (NP444_02000)	czcD	431820..432719 (-)	900	WP_256713406.1	CDF family zinc transporter CzcD	-
NP444_RS02005 (NP444_02005)	-	432873..433277 (+)	405	WP_003084560.1	NINE protein	-
NP444_RS02010 (NP444_02010)	-	433520..434893 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
NP444_RS02015 (NP444_02015)	-	434890..436074 (+)	1185	WP_256713407.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=712709 NP444_RS01995 WP_003100959.1 430664..431812(+) (pilU) [Pseudomonas aeruginosa strain NWRC-1223]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=712709 NP444_RS01995 WP_003100959.1 430664..431812(+) (pilU) [Pseudomonas aeruginosa strain NWRC-1223]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATTGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382