Detailed information NTG0711363

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MBA02_RS06595
• Genome accession: NZ_CP101656
• Coordinates: 1438096..1439244 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain L1a
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1433096..1444244

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MBA02_RS06570 (MBA02_006570)	-	1434247..1434543 (-)	297	WP_031655952.1	DUF167 domain-containing protein	-
MBA02_RS06575 (MBA02_006575)	-	1434540..1435133 (-)	594	WP_003084544.1	YggT family protein	-
MBA02_RS06580 (MBA02_006580)	proC	1435144..1435965 (-)	822	WP_003114890.1	pyrroline-5-carboxylate reductase	-
MBA02_RS06585 (MBA02_006585)	-	1435977..1436669 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
MBA02_RS06590 (MBA02_006590)	pilT	1436884..1437918 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
MBA02_RS06595 (MBA02_006595)	pilU	1438096..1439244 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
MBA02_RS06600 (MBA02_006600)	czcD	1439252..1440151 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
MBA02_RS06605 (MBA02_006605)	-	1440305..1440709 (+)	405	WP_003084560.1	NINE protein	-
MBA02_RS06610 (MBA02_006610)	-	1440952..1442325 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
MBA02_RS06615 (MBA02_006615)	-	1442322..1443506 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=711363 MBA02_RS06595 WP_003100959.1 1438096..1439244(+) (pilU) [Pseudomonas aeruginosa strain L1a]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=711363 MBA02_RS06595 WP_003100959.1 1438096..1439244(+) (pilU) [Pseudomonas aeruginosa strain L1a]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382