Detailed information NTG0711307

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: POF61_RS00425
• Genome accession: NZ_CP116709
• Coordinates: 66033..67031 (+)
• Length: 332 a.a.
• NCBI ID: WP_000196633.1
• Uniprot ID: Q3K3X8
• Organism: Streptococcus agalactiae strain CNCTC 10/84 sCas9revertant
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 61033..72031

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
POF61_RS00405 (POF61_00405)	-	61563..61820 (+)	258	WP_000590468.1	hypothetical protein	-
POF61_RS00410 (POF61_00410)	-	62185..63288 (+)	1104	WP_025194614.1	DUF6287 domain-containing protein	-
POF61_RS00415 (POF61_00415)	purB	63374..64672 (+)	1299	WP_000572895.1	adenylosuccinate lyase	-
POF61_RS00420 (POF61_00420)	comR	64825..65736 (+)	912	WP_000912100.1	helix-turn-helix domain-containing protein	Regulator
POF61_RS00425 (POF61_00425)	ruvB	66033..67031 (+)	999	WP_000196633.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
POF61_RS00430 (POF61_00430)	-	67183..67620 (+)	438	WP_000754816.1	low molecular weight protein-tyrosine-phosphatase	-
POF61_RS00435 (POF61_00435)	-	67627..68007 (+)	381	WP_000787701.1	hypothetical protein	-
POF61_RS00440 (POF61_00440)	-	68004..69782 (+)	1779	WP_001220911.1	acyltransferase family protein	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37573.90 Da
• Isoelectric Point: 4.5053

>NTDB_id=711307 POF61_RS00425 WP_000196633.1 66033..67031(+) (ruvB) [Streptococcus agalactiae strain CNCTC 10/84 sCas9revertant]
MTRFLDSDAMGDEELVERTLRPQYLREYIGQDKVKDQLKIFIEAAKLRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAIEKSGDLVAILNDLEPGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGAGETSRSVHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYEENDLTEIIERTADIFEMKITYEAASELARRSRGTPRIANRLLKRVRDYAQIMGDGLID
DNITDKALTMLDVDHEGLDYVDQKILRTMIEMYNGGPVGLGTLSVNIAEERDTVEDMYEPYLIQKGFIMRTRTGRVATDK
AYEHLGYQRFDK

Nucleotide

• Length: 999 bp

>NTDB_id=711307 POF61_RS00425 WP_000196633.1 66033..67031(+) (ruvB) [Streptococcus agalactiae strain CNCTC 10/84 sCas9revertant]
ATGACAAGATTTTTAGATAGTGATGCAATGGGTGACGAAGAATTGGTAGAACGTACACTTCGTCCGCAGTATTTAAGAGA
GTATATTGGACAAGATAAGGTTAAAGATCAGCTAAAAATATTTATTGAAGCTGCTAAATTGCGTGATGAGTCATTGGATC
ATGTGTTATTATTTGGCCCTCCTGGTTTAGGGAAAACAACCATGGCATTTGTAATTGCTAATGAGTTGGGTGTCAATCTC
AAACAAACATCAGGTCCCGCAATTGAAAAATCAGGGGATTTAGTAGCCATTTTAAATGATTTAGAACCAGGTGATGTTCT
TTTTATTGATGAAATTCATCGTATGCCGATGGCGGTTGAAGAGGTACTTTATAGTGCAATGGAAGACTTCTATATTGACA
TTATGATCGGTGCAGGAGAAACTAGTAGAAGTGTTCATTTAGATTTGCCGCCCTTTACCTTAATTGGTGCAACGACACGT
GCAGGTATGTTATCTAATCCCTTACGTGCTCGCTTTGGTATTACAGGGCATATGGAGTATTATGAAGAAAATGATTTGAC
AGAAATTATTGAGCGTACAGCAGACATTTTTGAAATGAAAATCACTTATGAAGCTGCTTCTGAATTAGCGCGTCGCAGTC
GTGGAACGCCACGTATCGCTAACCGTTTATTGAAACGTGTTCGAGATTATGCTCAAATCATGGGAGATGGTTTGATAGAT
GACAATATTACAGATAAAGCATTAACGATGTTAGATGTTGATCACGAGGGGCTTGATTACGTCGATCAAAAAATCTTAAG
AACCATGATTGAAATGTATAATGGAGGTCCTGTTGGTTTAGGAACTCTATCCGTTAATATTGCTGAAGAACGAGATACTG
TTGAAGACATGTACGAACCTTATTTAATTCAAAAAGGTTTTATTATGCGTACCCGTACCGGTCGTGTAGCTACGGATAAG
GCATACGAACATTTAGGTTATCAGCGATTTGATAAATAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• RuvB AAA lid domain (AAA_lid_4) (181-254)

3D structure

• AlphaFold DB: Q3K3X8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae TIGR4	90.06	100	0.901
ruvB	Streptococcus pneumoniae R6	89.759	100	0.898
ruvB	Streptococcus pneumoniae D39	89.759	100	0.898
ruvB	Bacillus subtilis subsp. subtilis str. 168	59.819	99.699	0.596
ruvB	Helicobacter pylori 26695	53.074	93.072	0.494
ruvB	Synechocystis sp. PCC 6803	50.479	94.277	0.476