Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   NMH04_RS12000 Genome accession   NZ_CP101609
Coordinates   2326502..2326795 (-) Length   97 a.a.
NCBI ID   WP_008342186.1    Uniprot ID   A0A5K1NAM4
Organism   Bacillus altitudinis strain 47     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2321502..2331795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMH04_RS11950 (NMH04_11895) sinR 2321666..2322001 (+) 336 WP_017357978.1 transcriptional regulator SinR Regulator
  NMH04_RS11955 (NMH04_11900) tasA 2322069..2322869 (-) 801 WP_008342195.1 biofilm matrix protein TasA -
  NMH04_RS11960 (NMH04_11905) sipW 2322928..2323500 (-) 573 WP_017357979.1 signal peptidase I SipW -
  NMH04_RS11965 (NMH04_11910) tapA 2323550..2324077 (-) 528 WP_061419578.1 amyloid fiber anchoring/assembly protein TapA -
  NMH04_RS11970 (NMH04_11915) - 2324330..2324656 (+) 327 WP_041506959.1 DUF3889 domain-containing protein -
  NMH04_RS11975 (NMH04_11920) - 2324691..2324885 (-) 195 WP_008342191.1 YqzE family protein -
  NMH04_RS11980 (NMH04_11925) comGG 2324946..2325329 (-) 384 WP_041506958.1 competence type IV pilus minor pilin ComGG -
  NMH04_RS11985 (NMH04_11930) comGF 2325326..2325823 (-) 498 WP_041506957.1 competence type IV pilus minor pilin ComGF -
  NMH04_RS11990 (NMH04_11935) comGE 2325765..2326079 (-) 315 WP_041506956.1 competence type IV pilus minor pilin ComGE -
  NMH04_RS11995 (NMH04_11940) comGD 2326063..2326509 (-) 447 WP_041506955.1 competence type IV pilus minor pilin ComGD -
  NMH04_RS12000 (NMH04_11945) comGC 2326502..2326795 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  NMH04_RS12005 (NMH04_11950) comGB 2326812..2327852 (-) 1041 WP_041506954.1 competence type IV pilus assembly protein ComGB -
  NMH04_RS12010 (NMH04_11955) comGA 2327833..2328903 (-) 1071 WP_024718839.1 competence type IV pilus ATPase ComGA Machinery gene
  NMH04_RS12015 (NMH04_11960) - 2329360..2330232 (-) 873 WP_041506953.1 STAS domain-containing protein -
  NMH04_RS12025 (NMH04_11970) - 2330457..2330837 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NMH04_RS12030 (NMH04_11975) - 2331050..2331292 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10728.62 Da        Isoelectric Point: 6.7081

>NTDB_id=710796 NMH04_RS12000 WP_008342186.1 2326502..2326795(-) (comGC) [Bacillus altitudinis strain 47]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQSIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIFIQNGDVKNE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=710796 NMH04_RS12000 WP_008342186.1 2326502..2326795(-) (comGC) [Bacillus altitudinis strain 47]
ATGAATGATAAAGGATTTACACTGTTAGAAATGTTAATTGTCATGCTTGTGATCTCCATTCTTTTATTGATCACCATTCC
AAATATCACTCGGCATCATCAAAGCATTCAAGCAAAGGGCTGTGAAGGGCTGAAATCAATGGTGAGTACACAGGTAATGG
CATATGAATTAGAGCATGACGGCAAGACTCCATCTTTAGCTGAATTAGAAAAAGAAGGCTATGTCAAAAAAGGATTAACT
TGTCCGAATGGAAAAGCAATTTTCATTCAAAACGGCGATGTGAAAAATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5K1NAM4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67
  comGC Staphylococcus aureus MW2

41.304

94.845

0.392
  comGC Staphylococcus aureus N315

41.304

94.845

0.392