Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NMH04_RS12010 Genome accession   NZ_CP101609
Coordinates   2327833..2328903 (-) Length   356 a.a.
NCBI ID   WP_024718839.1    Uniprot ID   -
Organism   Bacillus altitudinis strain 47     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2322833..2333903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMH04_RS11960 (NMH04_11905) sipW 2322928..2323500 (-) 573 WP_017357979.1 signal peptidase I SipW -
  NMH04_RS11965 (NMH04_11910) tapA 2323550..2324077 (-) 528 WP_061419578.1 amyloid fiber anchoring/assembly protein TapA -
  NMH04_RS11970 (NMH04_11915) - 2324330..2324656 (+) 327 WP_041506959.1 DUF3889 domain-containing protein -
  NMH04_RS11975 (NMH04_11920) - 2324691..2324885 (-) 195 WP_008342191.1 YqzE family protein -
  NMH04_RS11980 (NMH04_11925) comGG 2324946..2325329 (-) 384 WP_041506958.1 competence type IV pilus minor pilin ComGG -
  NMH04_RS11985 (NMH04_11930) comGF 2325326..2325823 (-) 498 WP_041506957.1 competence type IV pilus minor pilin ComGF -
  NMH04_RS11990 (NMH04_11935) comGE 2325765..2326079 (-) 315 WP_041506956.1 competence type IV pilus minor pilin ComGE -
  NMH04_RS11995 (NMH04_11940) comGD 2326063..2326509 (-) 447 WP_041506955.1 competence type IV pilus minor pilin ComGD -
  NMH04_RS12000 (NMH04_11945) comGC 2326502..2326795 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  NMH04_RS12005 (NMH04_11950) comGB 2326812..2327852 (-) 1041 WP_041506954.1 competence type IV pilus assembly protein ComGB -
  NMH04_RS12010 (NMH04_11955) comGA 2327833..2328903 (-) 1071 WP_024718839.1 competence type IV pilus ATPase ComGA Machinery gene
  NMH04_RS12015 (NMH04_11960) - 2329360..2330232 (-) 873 WP_041506953.1 STAS domain-containing protein -
  NMH04_RS12025 (NMH04_11970) - 2330457..2330837 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NMH04_RS12030 (NMH04_11975) - 2331050..2331292 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  NMH04_RS12035 (NMH04_11980) - 2331341..2331979 (-) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  NMH04_RS12040 (NMH04_11985) - 2332140..2332313 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  NMH04_RS12045 (NMH04_11990) - 2332436..2333788 (+) 1353 WP_061418470.1 IS1182 family transposase -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40366.72 Da        Isoelectric Point: 9.0449

>NTDB_id=710797 NMH04_RS12010 WP_024718839.1 2327833..2328903(-) (comGA) [Bacillus altitudinis strain 47]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEANL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=710797 NMH04_RS12010 WP_024718839.1 2327833..2328903(-) (comGA) [Bacillus altitudinis strain 47]
TTGTATGGTATTGAATATTTGGGGCAAGAGCTGCTAGAAGAGGCGTGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGATCATTGGCT
GTGATGCTAAGACGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCAACTGTAAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAGCGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACAGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGGAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGTTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCAGAAACAGCGAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACACTGCAC
GCAAAGAATGCAAAAGGAGCTCTTTATCGAATGCTCGAATTTGGTGTGACGATGAATGAACTCGAACAAACAATGGTTGC
CATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGGTTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAACCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.888

100

0.649