Detailed information NTG0710604

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: POF49_RS14610
• Genome accession: NZ_CP116680
• Coordinates: 3039778..3040896 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A086HYC5
• Organism: Acinetobacter baumannii Canada BC-5
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3034778..3045896

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
POF49_RS14600 (POF49_14600)	-	3037894..3038586 (-)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-
POF49_RS14605 (POF49_14605)	pilT	3038713..3039750 (+)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
POF49_RS14610 (POF49_14610)	pilU	3039778..3040896 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
POF49_RS14615 (POF49_14615)	fur	3040966..3041403 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
POF49_RS14620 (POF49_14620)	-	3041515..3041913 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
POF49_RS14625 (POF49_14625)	-	3041968..3042291 (-)	324	WP_000442585.1	RnfH family protein	-
POF49_RS14630 (POF49_14630)	-	3042291..3043358 (-)	1068	WP_000092403.1	hypothetical protein	-
POF49_RS14635 (POF49_14635)	-	3043511..3043966 (-)	456	WP_000782976.1	bacteriohemerythrin	-
POF49_RS14640 (POF49_14640)	-	3044219..3045067 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=710604 POF49_RS14610 WP_000347036.1 3039778..3040896(+) (pilU) [Acinetobacter baumannii Canada BC-5]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=710604 POF49_RS14610 WP_000347036.1 3039778..3040896(+) (pilU) [Acinetobacter baumannii Canada BC-5]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A086HYC5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392