Detailed information NTG0710523

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NNL25_RS01970
• Genome accession: NZ_CP101540
• Coordinates: 421041..422189 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain D-2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 416041..427189

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NNL25_RS01945 (NNL25_01945)	-	417192..417488 (-)	297	WP_031628073.1	DUF167 domain-containing protein	-
NNL25_RS01950 (NNL25_01950)	-	417485..418078 (-)	594	WP_003084544.1	YggT family protein	-
NNL25_RS01955 (NNL25_01955)	proC	418089..418910 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
NNL25_RS01960 (NNL25_01960)	-	418922..419614 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
NNL25_RS01965 (NNL25_01965)	pilT	419829..420863 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
NNL25_RS01970 (NNL25_01970)	pilU	421041..422189 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
NNL25_RS01975 (NNL25_01975)	czcD	422197..423096 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
NNL25_RS01980 (NNL25_01980)	-	423250..423654 (+)	405	WP_003084560.1	NINE protein	-
NNL25_RS01985 (NNL25_01985)	-	423897..425270 (+)	1374	WP_003160915.1	pyridoxal-phosphate dependent enzyme	-
NNL25_RS01990 (NNL25_01990)	-	425267..426451 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=710523 NNL25_RS01970 WP_003100959.1 421041..422189(+) (pilU) [Pseudomonas aeruginosa strain D-2]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=710523 NNL25_RS01970 WP_003100959.1 421041..422189(+) (pilU) [Pseudomonas aeruginosa strain D-2]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382