Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   K6J68_RS03265 Genome accession   NZ_AP018773
Coordinates   646116..646655 (-) Length   179 a.a.
NCBI ID   WP_044345365.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-III-2 isolate 13H39     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 641116..651655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J68_RS03235 (CHBNIII2_06220) fdx 641519..641860 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  K6J68_RS03240 (CHBNIII2_06230) iscX 641860..642054 (+) 195 WP_005626949.1 Fe-S cluster assembly protein IscX -
  K6J68_RS03245 (CHBNIII2_06240) - 642105..642719 (-) 615 WP_005686818.1 YfgM family protein -
  K6J68_RS03250 (CHBNIII2_06250) hisS 642737..644008 (-) 1272 WP_005686816.1 histidine--tRNA ligase -
  K6J68_RS03255 (CHBNIII2_06260) ispG 644018..645124 (-) 1107 WP_005686814.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  K6J68_RS03260 (CHBNIII2_06270) - 645133..646044 (-) 912 WP_005686812.1 RodZ family helix-turn-helix domain-containing protein -
  K6J68_RS03265 (CHBNIII2_06280) pilF/pilF2 646116..646655 (-) 540 WP_044345365.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  K6J68_RS03270 (CHBNIII2_06290) - 646756..647907 (-) 1152 WP_044345367.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  K6J68_RS03275 (CHBNIII2_06300) - 648154..649032 (+) 879 WP_005656360.1 D-alanyl-D-alanine carboxypeptidase family protein -
  K6J68_RS03280 (CHBNIII2_06310) - 649418..650278 (+) 861 WP_224056855.1 metal ABC transporter substrate-binding protein -
  K6J68_RS03285 (CHBNIII2_06320) - 650278..651198 (+) 921 WP_005630123.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20652.47 Da        Isoelectric Point: 9.2047

>NTDB_id=71038 K6J68_RS03265 WP_044345365.1 646116..646655(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-III-2 isolate 13H39]
MQTISKQLSAVIFPFIFSACVSQSVSNLDHQAAAKVRVELALSYLQQNNPQLAKINLDKALLHDKNYYLVHSALAHYYQQ
QGQIKNAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQTDTFENIALCAYSAKKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=71038 K6J68_RS03265 WP_044345365.1 646116..646655(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-III-2 isolate 13H39]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCTGTATCTAA
CTTAGATCATCAAGCTGCCGCCAAAGTGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCTACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAAAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAGCAAGCTCAACAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAACAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

93.296

100

0.933
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment