Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   XCCCN05v2_RS05395 Genome accession   NZ_CP101170
Coordinates   1286041..1287777 (+) Length   578 a.a.
NCBI ID   WP_228422809.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain CN05     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1281041..1292777
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XCCCN05v2_RS05365 coaE 1281822..1282445 (-) 624 WP_228422810.1 dephospho-CoA kinase -
  XCCCN05v2_RS05370 - 1282459..1283322 (-) 864 WP_223646361.1 A24 family peptidase -
  XCCCN05v2_RS05375 pilC 1283329..1284588 (-) 1260 WP_223646360.1 type II secretion system F family protein Machinery gene
  XCCCN05v2_RS05380 - 1284942..1285382 (+) 441 WP_223646359.1 pilin -
  XCCCN05v2_RS22255 - 1285541..1285947 (+) 407 Protein_1048 pilin -
  XCCCN05v2_RS05395 pilB 1286041..1287777 (+) 1737 WP_228422809.1 type IV-A pilus assembly ATPase PilB Machinery gene
  XCCCN05v2_RS05405 pilR 1288196..1289590 (-) 1395 WP_228422808.1 sigma-54 dependent transcriptional regulator Regulator
  XCCCN05v2_RS05410 - 1289799..1291409 (-) 1611 WP_228422807.1 HAMP domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62934.04 Da        Isoelectric Point: 5.6058

>NTDB_id=709094 XCCCN05v2_RS05395 WP_228422809.1 1286041..1287777(+) (pilB) [Xanthomonas campestris pv. campestris strain CN05]
MNQVSSSANLVGITGIARRLVQDGALEEAAARIAMEQATAAKVPLPQWFSDKKLVSGAQLAAANAVEFGMPLMDVSAFDA
NQNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASFGSSL
GNDDDEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKA
PVKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQ
KLFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQD
PDIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTL
PEHALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALM
KASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=709094 XCCCN05v2_RS05395 WP_228422809.1 1286041..1287777(+) (pilB) [Xanthomonas campestris pv. campestris strain CN05]
GTGAACCAAGTATCAAGTAGCGCTAATTTGGTCGGCATCACAGGTATAGCCCGTCGGCTTGTCCAAGATGGGGCCTTAGA
AGAAGCTGCTGCACGTATTGCGATGGAGCAGGCTACTGCAGCTAAAGTTCCGTTGCCACAATGGTTTTCTGATAAAAAAC
TAGTCTCCGGGGCTCAACTCGCTGCAGCCAACGCCGTTGAGTTCGGGATGCCGCTGATGGATGTGTCGGCGTTCGACGCC
AATCAGAACGCGGTCAAGCTGGTCAGTGAGGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCG
GTTGTTCGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGATATCAAGTTCCATACGAACCTGGTTGTCGAGC
CTATCCTGGTCGACGAGGACCAGATTCGTCGCACTTTGGAGCAGTGGCAGGCAAGTAACGCTTCGTTTGGTTCGTCGTTG
GGGAACGACGATGACGAGATGGGGGACCTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGT
CGATGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGG
ACATCCATTTCGAGCCCTATGAAGATGACTACCGGGTGCGGTTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCG
CCGGTGAAGCTCAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGATATTGCCGAGAAGCGGGTGCCGCA
GGACGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACCCTGTTCGGCG
AGAAGGTGGTGCTGCGTATCCTGGACGCCAGTGCGGCCAAGCTCGGTATCGAGAAGCTGGGCTATGAGGCGGACCAGCAG
AAGCTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTAACCGGGCCGACCGGTTCGGGCAAGACGGTGTC
GTTGTATACGGCGCTGGGCATCCTCAACGATGAGACGCGCAATATTTCCACGGCCGAGGATCCGGTCGAAATCCGCTTGC
CCGGCGTCAATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACGTTTGCGGCGGCGCTGCGCTCGTTCCTGCGTCAGGAC
CCGGACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGCCATAT
GGTGCTGTCGACACTGCACACCAACGATGCGCCGCAAACCATTGCGCGTCTGATGAACATGGGTATCGCGCCGTACAACA
TCACCTCGTCGGTGACGCTGGTCATCGCGCAGCGCCTGGCGCGGCGGCTGTGCAACAACTGCAAGCGCAAGTCGACGCTG
CCGGAACATGCGCTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTTGCGAACATCGAGCTGTATGAAGCGGTCGGTTGCGA
TGAGTGCACGGAAGGCTACAAGGGCCGTACCGGCATCTATCAGGTGATGCCGATGACCGATGAAATCGGCGCGATCGTAC
TGGAAGGCGGCAATGCGATGCAGATCGCCGAGGCTGCGCAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTGATG
AAGGCTTCGCACGGCGTGACCAGCCTGGCCGAAATCAATCGTGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(202-469)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

56.162

98.27

0.552
  pilB Acinetobacter baumannii D1279779

55.458

98.27

0.545
  pilB Legionella pneumophila strain ERS1305867

51.937

98.27

0.51
  pilF Neisseria gonorrhoeae MS11

49.12

98.27

0.483
  pilB Vibrio cholerae strain A1552

48.168

99.135

0.478
  pilB Vibrio campbellii strain DS40M4

46.046

98.443

0.453
  pilB Vibrio parahaemolyticus RIMD 2210633

46.333

96.713

0.448