Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   PIC88_RS01640 Genome accession   NZ_CP116188
Coordinates   364513..365751 (+) Length   412 a.a.
NCBI ID   WP_000815984.1    Uniprot ID   -
Organism   Escherichia coli strain DETEC-E223     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 359513..370751
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIC88_RS01615 (PIC88_01615) mrcA 359793..362345 (-) 2553 WP_048218044.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  PIC88_RS01620 (PIC88_01620) hofM 362465..363244 (+) 780 WP_032298081.1 DNA utilization protein HofM -
  PIC88_RS01625 (PIC88_01625) hofN 363244..363783 (+) 540 WP_001069320.1 DNA utilization protein HofN -
  PIC88_RS01630 (PIC88_01630) hofO 363767..364207 (+) 441 WP_001055768.1 DNA utilization protein HofO -
  PIC88_RS01635 (PIC88_01635) hofP 364197..364601 (+) 405 WP_001338740.1 DNA utilization protein HofP -
  PIC88_RS01640 (PIC88_01640) comE 364513..365751 (+) 1239 WP_000815984.1 DNA uptake porin HofQ Machinery gene
  PIC88_RS01645 (PIC88_01645) aroK 366152..366673 (+) 522 WP_000818618.1 shikimate kinase AroK -
  PIC88_RS01650 (PIC88_01650) aroB 366730..367818 (+) 1089 WP_000439849.1 3-dehydroquinate synthase -
  PIC88_RS01655 (PIC88_01655) damX 367910..369208 (+) 1299 WP_048218039.1 cell division protein DamX -
  PIC88_RS01660 (PIC88_01660) dam 369315..370151 (+) 837 WP_000742143.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44643.18 Da        Isoelectric Point: 6.3183

>NTDB_id=708875 PIC88_RS01640 WP_000815984.1 364513..365751(+) (comE) [Escherichia coli strain DETEC-E223]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNNNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPLFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=708875 PIC88_RS01640 WP_000815984.1 364513..365751(+) (comE) [Escherichia coli strain DETEC-E223]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAAGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGCCAGGAGGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATAACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCACTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGTTTGCTGGATCTTGAGCTTTCTGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCTAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCTGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTTGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  comE Haemophilus influenzae 86-028NP

36.927

100

0.391
  pilQ Vibrio campbellii strain DS40M4

38.242

100

0.391
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.53

100

0.376
  pilQ Vibrio cholerae strain A1552

37.53

100

0.376
  comE Glaesserella parasuis strain SC1401

35.952

100

0.367