Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   NGC85_RS01590 Genome accession   NZ_CP101115
Coordinates   342576..343220 (+) Length   214 a.a.
NCBI ID   WP_005059945.1    Uniprot ID   N9FQF6
Organism   Acinetobacter sp. Z1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 337576..348220
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGC85_RS01575 (NGC85_01575) - 337934..338752 (+) 819 WP_254804631.1 putative RNA methyltransferase -
  NGC85_RS01580 (NGC85_01580) ponA 338767..341355 (-) 2589 WP_254804632.1 penicillin-binding protein PBP1a -
  NGC85_RS01585 (NGC85_01585) comM 341518..342576 (+) 1059 WP_254804633.1 pilus assembly protein PilM Machinery gene
  NGC85_RS01590 (NGC85_01590) pilN 342576..343220 (+) 645 WP_005059945.1 PilN domain-containing protein Machinery gene
  NGC85_RS01595 (NGC85_01595) comO 343217..343951 (+) 735 WP_005052213.1 type 4a pilus biogenesis protein PilO Machinery gene
  NGC85_RS01600 (NGC85_01600) pilP 343954..344481 (+) 528 WP_169109293.1 pilus assembly protein PilP Machinery gene
  NGC85_RS01605 (NGC85_01605) pilQ 344498..346675 (+) 2178 WP_254804634.1 type IV pilus secretin PilQ Machinery gene
  NGC85_RS01610 (NGC85_01610) aroK 346686..347252 (+) 567 WP_155757334.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 214 a.a.        Molecular weight: 23849.22 Da        Isoelectric Point: 6.0168

>NTDB_id=708715 NGC85_RS01590 WP_005059945.1 342576..343220(+) (pilN) [Acinetobacter sp. Z1]
MAKINLLPWRDELREQRKKQFVAFCVGVAALGVASVFSGWMYFDHKLNDQEQANQLIVSTNQNLDTQLKALDGLQERRNA
IIERMKLIQGLQGQRPVTVRLVDELVRVTPPTMYITKFSRTGDKFSIEGKAESPNTVAELLRNLEASPWYRNAFMNSFLA
AEEKKDKTASSLVPRVEENYGSFVVTVDLGEIGTTEQADPALEAVASGTVGVAK

Nucleotide


Download         Length: 645 bp        

>NTDB_id=708715 NGC85_RS01590 WP_005059945.1 342576..343220(+) (pilN) [Acinetobacter sp. Z1]
ATGGCAAAGATTAATTTACTCCCTTGGCGTGATGAGCTAAGAGAACAAAGAAAGAAACAGTTTGTCGCATTTTGTGTTGG
GGTCGCTGCATTAGGGGTTGCATCTGTCTTTTCTGGGTGGATGTATTTTGATCATAAATTGAATGATCAAGAGCAGGCGA
ATCAACTGATTGTCAGTACAAATCAAAACTTAGATACACAATTAAAAGCGCTGGATGGTTTGCAAGAACGTCGTAATGCA
ATTATCGAGCGAATGAAATTAATCCAAGGTTTACAAGGTCAACGTCCTGTTACTGTTCGTTTGGTAGATGAGTTGGTTCG
AGTAACACCACCAACAATGTACATTACGAAATTTAGTCGGACAGGTGATAAATTTTCGATTGAAGGAAAAGCGGAAAGTC
CAAATACAGTCGCTGAATTACTACGTAATTTAGAGGCTTCGCCTTGGTATCGTAATGCGTTTATGAACTCGTTTTTAGCG
GCGGAAGAGAAAAAAGATAAAACCGCAAGCTCACTGGTTCCTCGCGTCGAAGAAAACTATGGAAGCTTTGTTGTCACTGT
AGATTTGGGGGAGATCGGTACGACGGAACAAGCTGATCCTGCATTAGAAGCCGTTGCATCAGGGACAGTAGGGGTGGCGA
AATGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-167)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9FQF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Acinetobacter baumannii D1279779

78.505

100

0.785
  comN Acinetobacter baylyi ADP1

77.949

91.121

0.71