Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   K6J54_RS03315 Genome accession   NZ_AP018766
Coordinates   666838..667377 (-) Length   179 a.a.
NCBI ID   WP_012054975.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-II-3 isolate 13H41     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 661838..672377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J54_RS03285 (CHBNII3_06240) fdx 662241..662582 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  K6J54_RS03290 (CHBNII3_06250) iscX 662582..662776 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  K6J54_RS03295 (CHBNII3_06260) - 662827..663441 (-) 615 WP_011272010.1 YfgM family protein -
  K6J54_RS03300 (CHBNII3_06270) hisS 663459..664730 (-) 1272 WP_038440737.1 histidine--tRNA ligase -
  K6J54_RS03305 (CHBNII3_06280) ispG 664740..665846 (-) 1107 WP_011272008.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  K6J54_RS03310 (CHBNII3_06290) - 665855..666766 (-) 912 WP_042593379.1 RodZ family helix-turn-helix domain-containing protein -
  K6J54_RS03315 (CHBNII3_06300) pilF/pilF2 666838..667377 (-) 540 WP_012054975.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  K6J54_RS03320 (CHBNII3_06310) - 667478..668629 (-) 1152 WP_042602892.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  K6J54_RS03325 (CHBNII3_06320) - 668876..669754 (+) 879 WP_042599462.1 D-alanyl-D-alanine carboxypeptidase family protein -
  K6J54_RS03330 (CHBNII3_06330) - 670145..671005 (+) 861 WP_223364826.1 metal ABC transporter substrate-binding protein -
  K6J54_RS03335 (CHBNII3_06340) - 671005..671925 (+) 921 WP_005630123.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20554.15 Da        Isoelectric Point: 8.2571

>NTDB_id=70794 K6J54_RS03315 WP_012054975.1 666838..667377(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-II-3 isolate 13H41]
MKTISKQLSAVIFPFIFSACVSQSASSLNHQAAAKARVELALSYLQQNNPQLAKINLDNALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=70794 K6J54_RS03315 WP_012054975.1 666838..667377(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-II-3 isolate 13H41]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTTATTTTTTCTGCTTGCGTTTCACAATCTGCATCTAG
CTTAAATCATCAAGCTGCCGCTAAAGCGCGCGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAACTTAGATAACGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCTCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATTGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

98.883

100

0.989
  pilF Glaesserella parasuis strain SC1401

44.134

100

0.441

Multiple sequence alignment