Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   PIB26_RS04100 Genome accession   NZ_CP115968
Coordinates   849966..851459 (+) Length   497 a.a.
NCBI ID   WP_000249329.1    Uniprot ID   -
Organism   Escherichia coli strain B-DidR     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 844966..856459
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIB26_RS04080 pilD 845614..846423 (+) 810 WP_000895873.1 prepilin peptidase PppA Machinery gene
  PIB26_RS04085 gspS2 846489..846899 (+) 411 WP_001305972.1 type II secretion system pilot lipoprotein GspS-beta -
  PIB26_RS04090 gspC 846917..847876 (+) 960 WP_014639304.1 type II secretion system protein GspC -
  PIB26_RS04095 gspD 847906..849966 (+) 2061 WP_000498829.1 type II secretion system secretin GspD -
  PIB26_RS04100 pilB/pilB1 849966..851459 (+) 1494 WP_000249329.1 type II secretion system ATPase GspE Machinery gene
  PIB26_RS04105 gspF 851459..852682 (+) 1224 WP_001173416.1 type II secretion system inner membrane protein GspF -
  PIB26_RS04110 gspG 852699..853154 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  PIB26_RS04115 gspH 853158..853721 (+) 564 WP_001115147.1 type II secretion system minor pseudopilin GspH -
  PIB26_RS04120 gspI 853718..854089 (+) 372 WP_000820109.1 type II secretion system minor pseudopilin GspI -
  PIB26_RS04125 gspJ 854086..854691 (+) 606 WP_023147221.1 type II secretion system minor pseudopilin GspJ -
  PIB26_RS04130 gspK 854688..855665 (+) 978 WP_000633200.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55230.39 Da        Isoelectric Point: 6.0089

>NTDB_id=706565 PIB26_RS04100 WP_000249329.1 849966..851459(+) (pilB/pilB1) [Escherichia coli strain B-DidR]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQALPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=706565 PIB26_RS04100 WP_000249329.1 849966..851459(+) (pilB/pilB1) [Escherichia coli strain B-DidR]
ATGGTGCCTGTAGCACAGGAAACCACTGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAACTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCAGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGCAAGCTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGTCTGGATATCGCCGAAAAGCGCGTACC
ACAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATCGACGTGCGCGTCTCCACCATGCCTTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCACGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACAGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGATCCCATCGAATACATGATTG
AAGGGATCGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTAGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCTTCACTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATTACGCGTTTGCAGGATATGGGGGTAGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTCCGTACGCTGTGCCCCGACTGCCGCCAGGCCCTGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCACAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGGCGTACTGCCATCCATGAGTTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGCATCCGCAGCAATGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.741

100

0.433
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Acinetobacter baumannii D1279779

46.883

80.684

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

45

80.483

0.362