Detailed information NTG0706071

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NLS69_RS02015
• Genome accession: NZ_CP100653
• Coordinates: 431678..432826 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain F13
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 426678..437826

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NLS69_RS01990 (NLS69_01990)	-	427829..428125 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
NLS69_RS01995 (NLS69_01995)	-	428122..428715 (-)	594	WP_003155373.1	YggT family protein	-
NLS69_RS02000 (NLS69_02000)	proC	428726..429547 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
NLS69_RS02005 (NLS69_02005)	-	429559..430251 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
NLS69_RS02010 (NLS69_02010)	pilT	430466..431500 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
NLS69_RS02015 (NLS69_02015)	pilU	431678..432826 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
NLS69_RS02020 (NLS69_02020)	czcD	432834..433733 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
NLS69_RS02025 (NLS69_02025)	-	433887..434291 (+)	405	WP_003084560.1	NINE protein	-
NLS69_RS02030 (NLS69_02030)	-	434534..435907 (+)	1374	WP_003160915.1	pyridoxal-phosphate dependent enzyme	-
NLS69_RS02035 (NLS69_02035)	-	435904..437088 (+)	1185	WP_003117897.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=706071 NLS69_RS02015 WP_003100959.1 431678..432826(+) (pilU) [Pseudomonas aeruginosa strain F13]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=706071 NLS69_RS02015 WP_003100959.1 431678..432826(+) (pilU) [Pseudomonas aeruginosa strain F13]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382