Detailed information NTG0706021

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: NLW79_RS13970
• Genome accession: NZ_CP100651
• Coordinates: 2695923..2696540 (-)
• Length: 205 a.a.
• NCBI ID: WP_127696752.1
• Uniprot ID: -
• Organism: Bacillus halotolerans strain MEC_B334
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2690923..2701540

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NLW79_RS13940 (NLW79_13940)	rpsT	2691226..2691492 (+)	267	WP_010335003.1	30S ribosomal protein S20	-
NLW79_RS13945 (NLW79_13945)	holA	2691507..2692550 (-)	1044	WP_106020122.1	DNA polymerase III subunit delta	-
NLW79_RS13950 (NLW79_13950)	-	2692590..2692739 (-)	150	WP_003229985.1	hypothetical protein	-
NLW79_RS13955 (NLW79_13955)	-	2692780..2692914 (+)	135	WP_010335005.1	YqzM family protein	-
NLW79_RS13960 (NLW79_13960)	comEC	2692953..2695283 (-)	2331	WP_254518317.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
NLW79_RS13965 (NLW79_13965)	-	2695287..2695856 (-)	570	WP_010335007.1	ComE operon protein 2	-
NLW79_RS13970 (NLW79_13970)	comEA	2695923..2696540 (-)	618	WP_127696752.1	helix-hairpin-helix domain-containing protein	Machinery gene
NLW79_RS13975 (NLW79_13975)	comER	2696624..2697445 (+)	822	WP_024122113.1	late competence protein ComER	-
NLW79_RS13980 (NLW79_13980)	-	2697510..2698253 (-)	744	WP_106020124.1	class I SAM-dependent methyltransferase	-
NLW79_RS13985 (NLW79_13985)	rsfS	2698250..2698606 (-)	357	WP_044154484.1	ribosome silencing factor	-
NLW79_RS13990 (NLW79_13990)	yqeK	2698624..2699184 (-)	561	WP_024122116.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
NLW79_RS13995 (NLW79_13995)	-	2699174..2699743 (-)	570	WP_010335014.1	nicotinate-nucleotide adenylyltransferase	-
NLW79_RS14000 (NLW79_14000)	yhbY	2699754..2700044 (-)	291	WP_024122118.1	ribosome assembly RNA-binding protein YhbY	-
NLW79_RS14005 (NLW79_14005)	aroE	2700044..2700880 (-)	837	WP_038954228.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 22018.82 Da
• Isoelectric Point: 4.7586

>NTDB_id=706021 NLW79_RS13970 WP_127696752.1 2695923..2696540(-) (comEA) [Bacillus halotolerans strain MEC_B334]
MNWLNQHKTIIMSVGSAAVLIAVLCLFFFSGNNKEPVKQVVSSEKENEEVKQEATKDKAEENIVIDIKGAVKHPGVYEMH
TGDRVSQAIEKAGGIGEKADETQVNLAELLQDGTVVYIPSKGEEAAQQAAGGAVQSSTGKETLVNINTATLEDLQNISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=706021 NLW79_RS13970 WP_127696752.1 2695923..2696540(-) (comEA) [Bacillus halotolerans strain MEC_B334]
TTGAATTGGTTGAATCAACATAAAACGATAATCATGTCAGTTGGCTCTGCTGCTGTTCTCATTGCAGTTCTTTGCCTCTT
TTTTTTCAGCGGAAATAACAAAGAGCCTGTAAAACAAGTTGTATCTAGTGAAAAGGAAAATGAGGAGGTCAAGCAGGAAG
CAACAAAAGACAAAGCGGAAGAAAATATTGTAATCGATATAAAAGGCGCTGTTAAGCATCCCGGCGTTTATGAAATGCAT
ACGGGAGACAGAGTATCGCAGGCTATTGAGAAAGCAGGCGGCATTGGTGAAAAAGCAGATGAAACGCAAGTGAATTTGGC
GGAACTTTTACAGGACGGTACAGTGGTGTATATACCGAGTAAGGGAGAAGAGGCGGCACAGCAAGCGGCCGGCGGTGCTG
TACAAAGCAGTACGGGGAAGGAGACTTTGGTCAATATCAACACAGCAACATTAGAGGATTTACAAAACATTTCGGGAGTA
GGGCCTTCAAAAGCCGAAGCGATTATTGCGTATCGGGAAGAGAACGGGCGTTTCCAGACAATCGAAGATATCACAAAGGT
TTCGGGAATTGGCGAAAAATCTTTTGAAAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-203)

• SLBB domain (SLBB) (64-119)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	77.184	100	0.776
comEA	Staphylococcus aureus N315	37.838	100	0.41
comEA	Staphylococcus aureus MW2	37.727	100	0.405
comEA	Latilactobacillus sakei subsp. sakei 23K	36.239	100	0.385
comEA	Streptococcus thermophilus LMD-9	37.264	100	0.385