Detailed information NTG0705838

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NLY38_RS22950
• Genome accession: NZ_CP100553
• Coordinates: 4877042..4878187 (-)
• Length: 381 a.a.
• NCBI ID: WP_012019966.1
• Uniprot ID: -
• Organism: Pseudomonas hydrolytica strain KHPS2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4872042..4883187

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NLY38_RS22930 (NLY38_22930)	ntrC	4872874..4874307 (+)	1434	WP_004373752.1	nitrogen regulation protein NR(I)	-
NLY38_RS22935 (NLY38_22935)	-	4874432..4874830 (-)	399	WP_195882054.1	TM2 domain-containing protein	-
NLY38_RS22940 (NLY38_22940)	-	4874978..4876189 (+)	1212	WP_195882053.1	putative DNA modification/repair radical SAM protein	-
NLY38_RS22945 (NLY38_22945)	-	4876189..4877076 (+)	888	WP_195882052.1	TIGR03915 family putative DNA repair protein	-
NLY38_RS22950 (NLY38_22950)	pilU	4877042..4878187 (-)	1146	WP_012019966.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NLY38_RS22955 (NLY38_22955)	pilT	4878275..4879309 (-)	1035	WP_004373763.1	type IV pilus twitching motility protein PilT	Machinery gene
NLY38_RS22960 (NLY38_22960)	-	4879385..4880077 (+)	693	WP_195882051.1	YggS family pyridoxal phosphate-dependent enzyme	-
NLY38_RS22965 (NLY38_22965)	proC	4880095..4880916 (+)	822	WP_195882050.1	pyrroline-5-carboxylate reductase	-
NLY38_RS22970 (NLY38_22970)	-	4880926..4881519 (+)	594	WP_004373770.1	YggT family protein	-
NLY38_RS22975 (NLY38_22975)	-	4881524..4881820 (+)	297	WP_254348178.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42459.72 Da
• Isoelectric Point: 6.5931

>NTDB_id=705838 NLY38_RS22950 WP_012019966.1 4877042..4878187(-) (pilU) [Pseudomonas hydrolytica strain KHPS2]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRIMPVTKNAMSPEQTRETVLGVMNEAQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTIDDLKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQNCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKGLMKRSTEQGMQTFDQA
LYNLYTQGEITYEDALLYADSANDLRLMIKLGSETDGDHLTSMAQGLALEVSEEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=705838 NLY38_RS22950 WP_012019966.1 4877042..4878187(-) (pilU) [Pseudomonas hydrolytica strain KHPS2]
ATGGAATTCGAAAAACTACTGCGCCTGATGGTCGAAAAAGGCGGCTCCGATCTGTTCATCACCGCTGGCGTGCCGCCGTC
GATGAAGGTCAATGGCCGGATCATGCCGGTGACCAAGAACGCCATGTCGCCGGAGCAGACCCGCGAAACGGTGCTGGGCG
TGATGAACGAGGCGCAGCGCCGCGATTTTGCCGAGAACCACGAGTGCAACTTCGCCATCAGTGCCCGTGGCATCGGTCGT
TTCCGCGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCCACCATCGA
TGACCTCAAGCTGCCGGAGATTCTCAAGAAGCTGGCGCTGACCAAGCGTGGCCTGGTGCTGTTCGTCGGTGCCACCGGTA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACAGCAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAATACATCCACCAGCACCAGAACTGCATCGTTACCCAGCGCGAGGTGGGCATCGACACCGAGTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGTCAGGCACCGGACGTGATCCTCATCGGTGAGGTGCGTACTCGCGAGACCATGGACCACG
CCGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGCCGACCGGCAGAACCAGGTGTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACCCCGGACGGCAAGGGCCGTCGCGCGGTGATCGAGGTGCTGATCAACACCCCGCTGGCTGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGGGTCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACATTCGACCAGGCG
CTGTACAACCTCTACACCCAGGGCGAGATCACTTACGAAGACGCGCTGCTCTACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAACCGACGGCGACCACCTGACCAGCATGGCCCAGGGCCTGGCGCTGGAGGTCAGCGAGG
AAGATCCGGGGCGTCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	92.388	100	0.924
pilU	Acinetobacter baylyi ADP1	64.146	93.701	0.601
pilU	Vibrio cholerae strain A1552	58.857	91.864	0.541
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.452	88.189	0.383