Detailed information NTG0705663

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PF553_RS04375
• Genome accession: NZ_CP115643
• Coordinates: 904522..905640 (-)
• Length: 372 a.a.
• NCBI ID: WP_024432390.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain 2022CK-00317
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 899522..910640

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PF553_RS04345 (PF553_04345)	-	900355..901203 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
PF553_RS04350 (PF553_04350)	-	901452..901907 (+)	456	WP_000782976.1	bacteriohemerythrin	-
PF553_RS04355 (PF553_04355)	-	902060..903127 (+)	1068	WP_029776981.1	membrane protein	-
PF553_RS04360 (PF553_04360)	-	903127..903450 (+)	324	WP_000442585.1	RnfH family protein	-
PF553_RS04365 (PF553_04365)	bamE	903505..903903 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
PF553_RS04370 (PF553_04370)	fur	904015..904452 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
PF553_RS04375 (PF553_04375)	pilU	904522..905640 (-)	1119	WP_024432390.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PF553_RS04380 (PF553_04380)	pilT	905668..906705 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
PF553_RS04385 (PF553_04385)	-	906832..907524 (+)	693	WP_024432389.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41889.11 Da
• Isoelectric Point: 6.6945

>NTDB_id=705663 PF553_RS04375 WP_024432390.1 904522..905640(-) (pilU) [Acinetobacter baumannii strain 2022CK-00317]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDFAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=705663 PF553_RS04375 WP_024432390.1 904522..905640(-) (pilU) [Acinetobacter baumannii strain 2022CK-00317]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
TCGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392