Detailed information NTG0705588

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PF550_RS04300
• Genome accession: NZ_CP115641
• Coordinates: 888289..889407 (-)
• Length: 372 a.a.
• NCBI ID: WP_024432390.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain 2022CK-00251
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 883289..894407

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PF550_RS04270 (PF550_04270)	-	884122..884970 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
PF550_RS04275 (PF550_04275)	-	885219..885674 (+)	456	WP_000782976.1	bacteriohemerythrin	-
PF550_RS04280 (PF550_04280)	-	885827..886894 (+)	1068	WP_029776981.1	membrane protein	-
PF550_RS04285 (PF550_04285)	-	886894..887217 (+)	324	WP_000442585.1	RnfH family protein	-
PF550_RS04290 (PF550_04290)	bamE	887272..887670 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
PF550_RS04295 (PF550_04295)	fur	887782..888219 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
PF550_RS04300 (PF550_04300)	pilU	888289..889407 (-)	1119	WP_024432390.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PF550_RS04305 (PF550_04305)	pilT	889435..890472 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
PF550_RS04310 (PF550_04310)	-	890599..891291 (+)	693	WP_024432389.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41889.11 Da
• Isoelectric Point: 6.6945

>NTDB_id=705588 PF550_RS04300 WP_024432390.1 888289..889407(-) (pilU) [Acinetobacter baumannii strain 2022CK-00251]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDFAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=705588 PF550_RS04300 WP_024432390.1 888289..889407(-) (pilU) [Acinetobacter baumannii strain 2022CK-00251]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
TCGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392