Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NLK52_RS12180 Genome accession   NZ_CP100436
Coordinates   2379713..2380783 (-) Length   356 a.a.
NCBI ID   WP_015483431.1    Uniprot ID   -
Organism   Bacillus subtilis strain MEC_B298     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2374713..2385783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLK52_RS12135 (NLK52_12135) tapA 2375193..2375954 (-) 762 WP_015384085.1 amyloid fiber anchoring/assembly protein TapA -
  NLK52_RS12140 (NLK52_12140) yqzG 2376224..2376550 (+) 327 WP_015384086.1 YqzG/YhdC family protein -
  NLK52_RS12145 (NLK52_12145) spoIITA 2376592..2376771 (-) 180 WP_003230176.1 YqzE family protein -
  NLK52_RS12150 (NLK52_12150) comGG 2376843..2377217 (-) 375 WP_014480253.1 ComG operon protein ComGG Machinery gene
  NLK52_RS12155 (NLK52_12155) comGF 2377218..2377601 (-) 384 WP_015384087.1 ComG operon protein ComGF Machinery gene
  NLK52_RS12160 (NLK52_12160) comGE 2377627..2377974 (-) 348 WP_015384088.1 ComG operon protein 5 Machinery gene
  NLK52_RS12165 (NLK52_12165) comGD 2377958..2378389 (-) 432 WP_015384089.1 comG operon protein ComGD Machinery gene
  NLK52_RS12170 (NLK52_12170) comGC 2378379..2378675 (-) 297 WP_014477332.1 comG operon protein ComGC Machinery gene
  NLK52_RS12175 (NLK52_12175) comGB 2378689..2379726 (-) 1038 WP_015483430.1 comG operon protein ComGB Machinery gene
  NLK52_RS12180 (NLK52_12180) comGA 2379713..2380783 (-) 1071 WP_015483431.1 competence protein ComGA Machinery gene
  NLK52_RS12185 (NLK52_12185) corA 2381193..2382146 (-) 954 WP_015483432.1 magnesium transporter CorA -
  NLK52_RS12190 (NLK52_12190) yqhB 2382289..2383617 (+) 1329 WP_003230150.1 hemolysin family protein -
  NLK52_RS12195 (NLK52_12195) rsbRD 2383670..2384506 (-) 837 WP_015384093.1 RsbT co-antagonist protein RsbRD -
  NLK52_RS12205 (NLK52_12205) mgsR 2384730..2385110 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  NLK52_RS12210 (NLK52_12210) yqgY 2385342..2385587 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=705439 NLK52_RS12180 WP_015483431.1 2379713..2380783(-) (comGA) [Bacillus subtilis strain MEC_B298]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYITTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=705439 NLK52_RS12180 WP_015483431.1 2379713..2380783(-) (comGA) [Bacillus subtilis strain MEC_B298]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATCGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGTGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCATTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGATCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTGACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTAGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGCCAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTTTACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATATAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

99.719

100

0.997
  pilF Thermus thermophilus HB27

36.827

99.157

0.365