Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EP379_RS14220 Genome accession   NZ_AP018724
Coordinates   2840796..2841869 (-) Length   357 a.a.
NCBI ID   WP_127478434.1    Uniprot ID   -
Organism   Sulfurivermis fontis strain JG42     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2817362..2840796 2840796..2841869 flank 0


Gene organization within MGE regions


Location: 2817362..2841869
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EP379_RS14105 - 2817362..2818201 (+) 840 WP_127478413.1 cytochrome c -
  EP379_RS14110 - 2818477..2819652 (+) 1176 WP_127478414.1 lysozyme inhibitor LprI family protein -
  EP379_RS14115 - 2819775..2820143 (-) 369 WP_127478415.1 hypothetical protein -
  EP379_RS16880 - 2820421..2820906 (+) 486 WP_127478416.1 RNA-binding protein -
  EP379_RS14125 - 2820953..2821723 (+) 771 WP_127478417.1 zinc-dependent peptidase -
  EP379_RS14130 nikR 2821733..2822155 (-) 423 WP_127478418.1 nickel-responsive transcriptional regulator NikR -
  EP379_RS14135 - 2822218..2822994 (-) 777 WP_127478419.1 energy-coupling factor ABC transporter ATP-binding protein -
  EP379_RS14140 - 2822987..2823538 (-) 552 WP_127478420.1 ABC transporter permease -
  EP379_RS14145 - 2823535..2824179 (-) 645 WP_127478421.1 energy-coupling factor ABC transporter permease -
  EP379_RS14150 - 2824179..2825159 (-) 981 WP_127478422.1 transporter -
  EP379_RS14155 - 2825156..2825575 (-) 420 WP_127478423.1 hypothetical protein -
  EP379_RS14160 - 2825572..2826243 (-) 672 WP_172600499.1 DUF4198 domain-containing protein -
  EP379_RS14165 - 2826383..2827693 (+) 1311 WP_127478425.1 NAD(P)/FAD-dependent oxidoreductase -
  EP379_RS14170 - 2827993..2830653 (+) 2661 WP_127478426.1 glycosyltransferase -
  EP379_RS14175 - 2830721..2831434 (+) 714 WP_127478427.1 pseudouridine synthase -
  EP379_RS14180 - 2831569..2832495 (+) 927 WP_127478428.1 hypothetical protein -
  EP379_RS14185 rrtA 2832467..2833045 (-) 579 WP_172600500.1 rhombosortase -
  EP379_RS14190 - 2833197..2835662 (-) 2466 WP_232023918.1 glycogen/starch/alpha-glucan phosphorylase -
  EP379_RS14195 aroK 2836270..2836818 (-) 549 WP_127478431.1 shikimate kinase AroK -
  EP379_RS14200 pilQ 2836845..2838995 (-) 2151 WP_232023919.1 type IV pilus secretin PilQ -
  EP379_RS14205 - 2839016..2839564 (-) 549 WP_172600501.1 pilus assembly protein PilP -
  EP379_RS14210 - 2839585..2840232 (-) 648 WP_127478939.1 type 4a pilus biogenesis protein PilO -
  EP379_RS14215 - 2840242..2840796 (-) 555 WP_127478433.1 PilN domain-containing protein -
  EP379_RS14220 pilM 2840796..2841869 (-) 1074 WP_127478434.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 38283.73 Da        Isoelectric Point: 4.5073

>NTDB_id=70524 EP379_RS14220 WP_127478434.1 2840796..2841869(-) (pilM) [Sulfurivermis fontis strain JG42]
MFSIAQLLQGSKPSILGLDISTTAVKLLELSKSGDRYRVESYAVEPLPPNSVIEKNISDVDAVGEAIKRAVKRSGSKAKH
AAAAVAGSAVITKVISMPANLSDAEMESQIQLEADQYIPYPLEEVNLDFQVLGPSDSDATRVDVLLAASRSENVDVRVAA
IELAGLTAKVIDVEAYAMENAFTLIAAQLPERGVDKTIAVVDVGATMTTLNVLHDLKTIYTREQVFGGKQLTEEIQRRYG
LSYEEAGMAKRQGGLPDNYVPEVLDPFKEAMAQQVSRSLQFFFSSSQFNSVDQIVLAGGSASITGVDELIEAKLGVPTMV
ANPFTNMSIAPRIKAQVLSNDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=70524 EP379_RS14220 WP_127478434.1 2840796..2841869(-) (pilM) [Sulfurivermis fontis strain JG42]
ATGTTCTCGATAGCCCAGCTCTTGCAAGGAAGTAAGCCATCGATACTCGGACTCGATATCAGCACGACTGCGGTCAAGCT
GCTGGAGCTGAGCAAGAGTGGCGACCGTTACCGGGTGGAAAGCTATGCGGTCGAACCGCTGCCACCTAACTCCGTGATCG
AGAAGAATATTTCCGATGTTGATGCGGTTGGCGAGGCCATCAAGCGGGCGGTCAAGCGCTCCGGCAGCAAGGCCAAGCAT
GCCGCCGCCGCCGTAGCCGGCTCGGCGGTAATCACCAAGGTCATTTCCATGCCCGCCAACCTCTCGGATGCCGAGATGGA
AAGCCAGATCCAACTGGAGGCGGATCAGTACATTCCCTACCCCCTGGAGGAAGTAAACCTGGATTTCCAGGTTCTTGGCC
CTTCGGACAGTGACGCGACCCGCGTCGATGTCTTGCTGGCAGCCTCACGCAGCGAAAACGTGGATGTGCGCGTGGCAGCA
ATCGAACTGGCGGGCCTGACTGCCAAGGTCATCGATGTCGAGGCCTACGCCATGGAAAATGCCTTCACCCTGATTGCGGC
GCAACTGCCGGAACGGGGTGTGGACAAGACCATTGCCGTGGTCGACGTCGGTGCGACGATGACCACGCTTAACGTCCTGC
ACGACCTCAAGACGATTTACACCCGCGAACAGGTGTTCGGCGGCAAGCAACTGACCGAGGAAATCCAGCGCCGCTACGGT
CTCTCCTACGAGGAAGCTGGCATGGCCAAGCGTCAGGGCGGCTTGCCGGACAACTACGTTCCTGAAGTGCTGGACCCGTT
CAAGGAGGCGATGGCCCAGCAGGTCAGCCGCTCACTCCAGTTTTTCTTCTCATCCAGCCAGTTCAACAGTGTTGATCAAA
TCGTATTGGCCGGTGGTTCCGCCTCCATAACCGGCGTCGATGAGTTGATCGAGGCCAAACTGGGAGTGCCGACCATGGTG
GCAAATCCCTTTACCAACATGAGTATCGCGCCCAGGATCAAGGCTCAGGTGCTGAGTAATGACGCTCCGGCCCTGATGAT
TGCCTGTGGCCTGGCCCTGCGGAGCTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.114

98.599

0.543
  comM Acinetobacter nosocomialis M2

54.83

98.599

0.541
  comM Acinetobacter baylyi ADP1

53.125

98.599

0.524
  pilM Legionella pneumophila strain ERS1305867

48.295

98.599

0.476

Multiple sequence alignment