Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PCP56_RS05420 Genome accession   NZ_CP115481
Coordinates   1166006..1166629 (-) Length   207 a.a.
NCBI ID   WP_003116135.1    Uniprot ID   A0A0H2ZIF6
Organism   Pseudomonas aeruginosa strain F002     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1161006..1171629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP56_RS05400 (PCP56_05405) aroB 1161613..1162719 (-) 1107 WP_003146400.1 3-dehydroquinate synthase -
  PCP56_RS05405 (PCP56_05410) aroK 1162769..1163287 (-) 519 WP_003095833.1 shikimate kinase AroK -
  PCP56_RS05410 (PCP56_05415) pilQ 1163299..1165431 (-) 2133 WP_003116137.1 type 4a pilus secretin PilQ Machinery gene
  PCP56_RS05415 (PCP56_05420) comL/pilP 1165485..1166009 (-) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  PCP56_RS05420 (PCP56_05425) pilO 1166006..1166629 (-) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO Machinery gene
  PCP56_RS05425 (PCP56_05430) pilN 1166626..1167222 (-) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  PCP56_RS05430 (PCP56_05435) pilM 1167222..1168287 (-) 1066 Protein_1063 type IV pilus assembly protein PilM -
  PCP56_RS05435 (PCP56_05440) - 1168472..1170943 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22958.35 Da        Isoelectric Point: 4.9107

>NTDB_id=704871 PCP56_RS05420 WP_003116135.1 1166006..1166629(-) (pilO) [Pseudomonas aeruginosa strain F002]
MSLASSLESLRKIDVNDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLRDLQTQLEQQAAQEESLKQQFATKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPASPDNTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=704871 PCP56_RS05420 WP_003116135.1 1166006..1166629(-) (pilO) [Pseudomonas aeruginosa strain F002]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTCCGTGACT
TGCAAACACAGCTCGAACAGCAGGCTGCGCAAGAAGAATCGCTCAAGCAGCAATTTGCTACCAAAGCGTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAAGAGATGGAGGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGTCTGGGCAGCGGCCTGGAGTTCGAGGAGATCAAGCTGCTCC
CTGAAGTCACTCAGCAGTTCTATATCGAGCTGCCGATCCAGATCAGCGTGGTAGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTTACCCTGCATGACTTCGAGATCAAGCCGGCTTCGCCCGATAACACCTC
GAAGCTGCGCATGAGCATCCTGGCCAAGACCTACCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZIF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

45.596

93.237

0.425
  pilO Acinetobacter baumannii D1279779

45.789

91.787

0.42
  comO/pilO Acinetobacter baylyi ADP1

43.617

90.821

0.396