Detailed information NTG0704823

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: NKI27_RS16530
• Genome accession: NZ_CP100390
• Coordinates: 3667249..3668313 (-)
• Length: 354 a.a.
• NCBI ID: WP_265047130.1
• Uniprot ID: -
• Organism: Alkalimarinus alittae strain A2M4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3662249..3673313

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NKI27_RS16510 (NKI27_16480)	pilQ	3663412..3665517 (-)	2106	WP_265047126.1	type IV pilus secretin PilQ	Machinery gene
NKI27_RS16515 (NKI27_16485)	-	3665533..3666072 (-)	540	WP_265047127.1	pilus assembly protein PilP	-
NKI27_RS16520 (NKI27_16490)	-	3666069..3666689 (-)	621	WP_265047128.1	type 4a pilus biogenesis protein PilO	-
NKI27_RS16525 (NKI27_16495)	-	3666686..3667249 (-)	564	WP_265047129.1	PilN domain-containing protein	-
NKI27_RS16530 (NKI27_16500)	pilM	3667249..3668313 (-)	1065	WP_265047130.1	pilus assembly protein PilM	Machinery gene
NKI27_RS16535 (NKI27_16505)	-	3668491..3671010 (+)	2520	WP_265047131.1	penicillin-binding protein 1A	-
NKI27_RS16540 (NKI27_16510)	-	3671048..3671851 (-)	804	WP_265047132.1	endonuclease/exonuclease/phosphatase family protein	-
NKI27_RS16545 (NKI27_16515)	-	3671991..3672725 (-)	735	WP_265047133.1	thiol:disulfide interchange protein DsbA/DsbL	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38080.98 Da
• Isoelectric Point: 4.1363

>NTDB_id=704823 NKI27_RS16530 WP_265047130.1 3667249..3668313(-) (pilM) [Alkalimarinus alittae strain A2M4]
MFGLIGKKANTLLGIDISSTSVKLLEIGKNGNRYRVESYAVEPLPPNAVVEKNINDAEAVGEVIKRVVAKSRTSSKQAAV
AVAGSAVITKVIQMAAGLSEQELENQITVEADQYIPYPLDEVSIDFEVQGPSEQNPDQVDVLLAACRKENVELREDSLEI
GGLEAKVVDVEAYAMERAFELIDAQMDADADERTVAVVDIGATMTTLSVLADGKTIYTREQLFGGKQLTEEIQRRYGLSQ
EEAGLAKKQGGLPDDYDMEVLEPFKEAVVQQVARSLQFFFSSSQYNEVDVVVLAGGTASTSGIAELVQDKVGTTTIVANP
FADMALNSRVNASALSNDAPSLMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=704823 NKI27_RS16530 WP_265047130.1 3667249..3668313(-) (pilM) [Alkalimarinus alittae strain A2M4]
GTGTTTGGGTTAATTGGAAAAAAAGCAAATACGCTACTTGGGATTGATATCAGCTCAACTTCGGTTAAGTTGCTTGAAAT
CGGAAAAAACGGTAATCGCTATAGGGTTGAAAGCTATGCTGTTGAACCGTTGCCGCCCAATGCTGTCGTAGAAAAAAATA
TCAATGATGCTGAAGCTGTTGGTGAAGTGATTAAGCGCGTTGTCGCTAAATCCAGAACAAGTTCCAAGCAAGCTGCTGTT
GCAGTGGCTGGATCTGCTGTTATTACCAAGGTTATTCAAATGGCTGCTGGGCTTAGTGAGCAGGAGCTTGAAAACCAGAT
AACAGTTGAAGCTGATCAGTATATTCCTTATCCGCTCGATGAAGTGTCAATCGACTTTGAAGTTCAAGGGCCGTCAGAAC
AAAATCCAGACCAAGTGGATGTTTTGCTTGCTGCATGCAGAAAGGAAAACGTTGAGCTTAGGGAGGATTCACTGGAAATT
GGCGGACTTGAAGCGAAGGTCGTTGATGTTGAAGCTTATGCGATGGAGCGTGCATTTGAGCTAATCGATGCTCAAATGGA
TGCCGACGCAGATGAGCGCACGGTTGCGGTTGTTGATATTGGTGCAACGATGACAACATTGAGTGTGCTTGCAGATGGCA
AAACCATCTACACTCGAGAGCAGTTATTCGGTGGCAAGCAACTGACAGAAGAGATACAACGGCGATATGGTTTGTCTCAA
GAAGAAGCGGGCTTAGCCAAAAAACAAGGGGGGCTTCCGGATGATTATGATATGGAAGTGCTAGAGCCCTTTAAAGAAGC
TGTTGTTCAACAAGTTGCGCGCTCGTTACAGTTTTTCTTTTCGTCAAGCCAGTATAATGAGGTTGATGTAGTGGTTCTTG
CCGGTGGTACGGCTTCCACAAGTGGAATTGCGGAGTTGGTGCAAGACAAAGTAGGCACGACAACGATTGTCGCAAATCCG
TTTGCAGATATGGCGTTAAACTCAAGAGTTAATGCGTCTGCCCTAAGTAATGATGCGCCATCGTTGATGATCGCGTGTGG
ATTGGCGATGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.932	100	0.559
comM	Acinetobacter nosocomialis M2	55.932	100	0.559
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	45.763	100	0.458