Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NKI27_RS16510 Genome accession   NZ_CP100390
Coordinates   3663412..3665517 (-) Length   701 a.a.
NCBI ID   WP_265047126.1    Uniprot ID   -
Organism   Alkalimarinus alittae strain A2M4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3658412..3670517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NKI27_RS16500 (NKI27_16470) aroK 3659160..3659696 (-) 537 WP_265047124.1 shikimate kinase AroK -
  NKI27_RS16505 (NKI27_16475) - 3660043..3661731 (+) 1689 WP_265047125.1 Ig-like domain-containing protein -
  NKI27_RS16510 (NKI27_16480) pilQ 3663412..3665517 (-) 2106 WP_265047126.1 type IV pilus secretin PilQ Machinery gene
  NKI27_RS16515 (NKI27_16485) - 3665533..3666072 (-) 540 WP_265047127.1 pilus assembly protein PilP -
  NKI27_RS16520 (NKI27_16490) - 3666069..3666689 (-) 621 WP_265047128.1 type 4a pilus biogenesis protein PilO -
  NKI27_RS16525 (NKI27_16495) - 3666686..3667249 (-) 564 WP_265047129.1 PilN domain-containing protein -
  NKI27_RS16530 (NKI27_16500) pilM 3667249..3668313 (-) 1065 WP_265047130.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75504.99 Da        Isoelectric Point: 4.7694

>NTDB_id=704822 NKI27_RS16510 WP_265047126.1 3663412..3665517(-) (pilQ) [Alkalimarinus alittae strain A2M4]
MNILYAEIDNKQLRLKNNMYYRALMLLGALCFSLFSIQANAVVLKDISFASLAGDRTEITLAFDGTPPEPKGYTIEKPAR
IALDLAGVTNALSAKYHSLGVGNAQGLTVIGAKDRTRLIVNLVELVPYASTVDGNNIILMVGSADAPKASSTGTMASKPS
KARAAAKAAVTDVDFRRGEKGDGQIVLTLSSPKIDVDMSEEGGLIKLSLAGVDLPEGLQRRLDVADFATPVKRIDSKLGD
NGVEITIKSEGNYDYLAYQADNQFTISVEELTAQEVDQRKKDKFPYTGEKLSLNFQNIEVRSVLQLIADFTGLNLVASDT
VGGQITLRLQNVPWDQALELVLKTKGLDKRKIGNVLLVAPADEIAAREKLELETNKQVAALAPVRLEVVQINYAKAQDIV
ALLQADKELISGRGFISSDERTNTISVRETADKQEQIRRLIATWDIPVRQVLIEARIVKARTNIASDLGIQWGGAVVDSG
SNHLGFGGGSYETTSQVSQGQAITFPGANMVDLGVTKAGASSFALGFTNDHVLLQMELSALESDGRGEVVAQPKIVTADR
QTATIKSGEEIPYQEASSSGATSVSFKEAVLSLEVTPQITPDGKVIMDLKINQDNRGEVTAGIPSIETNEITTQVLVGNG
ETVVLGGIFQTEVGTTITKTPFLGDIPYLGTLFRRTENIDERAELLIFITPKLLNRGLVDN

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=704822 NKI27_RS16510 WP_265047126.1 3663412..3665517(-) (pilQ) [Alkalimarinus alittae strain A2M4]
ATGAATATTTTATATGCTGAAATCGATAATAAACAATTGAGGTTGAAGAATAATATGTATTATCGGGCGTTAATGCTGTT
AGGTGCACTCTGCTTTTCTCTATTTTCAATTCAGGCTAACGCTGTTGTATTGAAAGATATTAGTTTTGCTTCGTTAGCTG
GAGACAGAACTGAAATTACATTGGCTTTTGACGGTACCCCGCCTGAACCAAAGGGATACACAATTGAAAAGCCAGCTCGC
ATCGCGCTGGACTTAGCTGGCGTTACTAACGCTTTAAGCGCTAAATACCATAGTTTGGGTGTGGGTAATGCGCAGGGACT
AACGGTTATAGGGGCTAAAGATAGAACGCGTCTTATCGTGAATCTTGTGGAGCTAGTGCCGTATGCATCAACAGTTGATG
GTAATAATATTATTTTAATGGTTGGCTCAGCTGATGCGCCTAAGGCGTCTTCAACTGGAACAATGGCTAGCAAGCCTTCA
AAGGCTCGAGCAGCAGCTAAGGCCGCGGTGACTGATGTTGATTTTAGACGCGGTGAGAAAGGCGATGGACAGATTGTTTT
AACCCTGTCTTCACCTAAAATTGACGTTGATATGTCTGAAGAAGGTGGACTGATTAAGTTGTCACTAGCAGGTGTTGATC
TTCCTGAAGGGCTACAGCGTCGTCTAGATGTTGCTGACTTTGCCACACCGGTAAAGAGAATTGACTCAAAACTAGGTGAT
AATGGCGTTGAAATTACAATTAAGTCTGAAGGTAATTATGACTACCTCGCTTACCAAGCAGACAACCAGTTCACCATCAG
TGTCGAAGAGTTAACCGCTCAAGAAGTGGATCAGCGTAAAAAGGATAAATTCCCTTATACCGGTGAAAAGCTATCGCTTA
ACTTTCAAAACATTGAAGTTCGGTCTGTATTACAGTTGATTGCTGACTTTACTGGTCTTAACTTAGTAGCAAGTGACACT
GTCGGCGGTCAAATTACATTAAGACTTCAAAATGTGCCTTGGGATCAGGCGCTTGAGTTAGTGCTTAAAACCAAGGGGCT
TGATAAGCGTAAGATTGGTAACGTGCTGTTGGTGGCGCCTGCTGATGAAATCGCAGCAAGAGAAAAGCTAGAGCTAGAAA
CTAACAAGCAAGTAGCAGCCCTTGCGCCAGTTAGACTTGAAGTGGTGCAAATTAATTATGCAAAAGCTCAAGATATTGTT
GCGCTTTTACAGGCAGATAAAGAGCTGATCTCGGGTCGAGGTTTTATCTCTTCTGACGAACGAACTAATACAATAAGTGT
GCGAGAAACTGCAGATAAGCAAGAACAAATCCGACGCTTAATAGCAACTTGGGATATTCCGGTTCGCCAAGTGCTTATTG
AGGCGCGCATCGTTAAGGCAAGGACCAATATTGCCAGTGATCTAGGAATTCAGTGGGGGGGCGCTGTTGTGGACTCAGGC
TCTAACCATCTTGGTTTTGGTGGTGGCAGTTATGAAACTACATCTCAGGTATCTCAAGGCCAAGCTATAACGTTCCCTGG
TGCCAATATGGTTGATTTGGGAGTGACTAAAGCAGGTGCATCTTCATTTGCTTTAGGCTTTACAAATGATCACGTGCTGC
TTCAAATGGAATTGTCGGCTCTTGAGTCGGATGGCCGTGGAGAGGTGGTTGCACAGCCTAAAATTGTTACCGCAGATCGT
CAGACAGCTACGATTAAATCGGGTGAAGAGATACCTTACCAAGAAGCTTCGTCAAGTGGTGCAACTTCAGTTTCTTTTAA
GGAGGCTGTGTTGAGTCTTGAGGTTACGCCTCAAATTACCCCAGACGGTAAAGTAATCATGGATTTAAAGATTAATCAAG
ATAACCGTGGTGAAGTGACTGCAGGGATACCGAGTATTGAAACCAATGAGATCACGACTCAAGTATTAGTTGGCAATGGT
GAAACGGTTGTTCTTGGCGGCATCTTTCAGACGGAAGTTGGGACGACTATAACCAAGACTCCATTTTTAGGGGATATTCC
TTACTTAGGCACGTTGTTTAGGCGCACAGAGAATATTGATGAAAGAGCTGAGCTGCTAATATTTATCACGCCTAAGCTGT
TGAATAGAGGGTTGGTTGATAACTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(540-694)

Secretin and TonB N terminus short domain (STN)

(314-360)

Bacterial type II/III secretion system short domain (Secretin_N)

(387-450)

AMIN domain (AMIN)

(54-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

50.072

99.715

0.499
  pilQ Acinetobacter baumannii strain A118

39.946

100

0.419
  pilQ Acinetobacter baumannii D1279779

39.946

100

0.419
  comQ Acinetobacter baylyi ADP1

40.506

100

0.411
  pilQ Legionella pneumophila strain Lp02

41.256

95.435

0.394
  pilQ Legionella pneumophila strain ERS1305867

41.168

95.292

0.392