Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NHM04_RS07700 Genome accession   NZ_CP100350
Coordinates   1742717..1743784 (-) Length   355 a.a.
NCBI ID   WP_254266400.1    Uniprot ID   A0A9J7ABD1
Organism   Gilvimarinus sp. DA14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1737717..1748784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHM04_RS07675 (NHM04_07675) aroK 1738344..1738853 (-) 510 WP_254266395.1 shikimate kinase AroK -
  NHM04_RS07680 (NHM04_07680) pilQ 1738857..1740968 (-) 2112 WP_254266396.1 type IV pilus secretin PilQ family protein -
  NHM04_RS07685 (NHM04_07685) - 1741005..1741535 (-) 531 WP_254266397.1 pilus assembly protein PilP -
  NHM04_RS07690 (NHM04_07690) - 1741525..1742136 (-) 612 WP_254266398.1 type 4a pilus biogenesis protein PilO -
  NHM04_RS07695 (NHM04_07695) - 1742136..1742717 (-) 582 WP_254266399.1 PilN domain-containing protein -
  NHM04_RS07700 (NHM04_07700) pilM 1742717..1743784 (-) 1068 WP_254266400.1 pilus assembly protein PilM Machinery gene
  NHM04_RS07705 (NHM04_07705) - 1743940..1746381 (+) 2442 WP_254266401.1 penicillin-binding protein 1A -
  NHM04_RS07710 (NHM04_07710) rpmE 1746459..1746680 (-) 222 WP_254266402.1 50S ribosomal protein L31 -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 38701.80 Da        Isoelectric Point: 4.1158

>NTDB_id=704695 NHM04_RS07700 WP_254266400.1 1742717..1743784(-) (pilM) [Gilvimarinus sp. DA14]
MGVFGFLDKKTKPVLGVDISSTSVKLLELSRSGDRYRVETYAVRALPPNAVVEKNINDPVAVAEVIRTLVKQSKTKVKQA
AVSVAGSAVITKVIEQPAGLSDDDLENRIYLEADQYIPFDTDEVSIDFEVVGESPNDPEMVEVLLAACRTENVDSRVEAL
QGAGLEEKVVDVEAYAMERAFELVLEQLEDQDNQVVAVLDVGATMTTLSVLVDGRTIYTREQLFGGRQLTEEIQRRYGLS
MEEAGLAKKQGGLPDDYQSEVLEPFKEAVIQQITRSLQFFFSSSEYNDVDYIVLAGGVASMEGLVELIEEKLGTQTLVAN
PFANMSVASRVNAAALANDAPSLMVATGLAMRSFD

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=704695 NHM04_RS07700 WP_254266400.1 1742717..1743784(-) (pilM) [Gilvimarinus sp. DA14]
ATGGGCGTTTTCGGATTCTTGGATAAAAAGACCAAGCCGGTGCTGGGGGTCGATATAAGCTCTACTTCGGTCAAGCTTCT
GGAGTTGAGTCGCAGCGGGGATCGTTACCGGGTAGAAACCTATGCGGTGCGTGCTCTGCCTCCCAACGCGGTGGTCGAAA
AAAACATTAATGACCCGGTGGCGGTGGCGGAAGTTATCCGCACTTTGGTAAAGCAGTCGAAAACCAAAGTTAAACAGGCC
GCTGTTTCGGTAGCTGGTTCTGCGGTTATCACCAAAGTCATTGAGCAGCCCGCTGGCTTGAGTGATGACGATCTGGAAAA
TCGCATCTACTTAGAAGCCGATCAATACATTCCTTTCGATACCGACGAAGTCTCCATTGATTTTGAGGTGGTGGGCGAGT
CTCCCAACGACCCGGAAATGGTCGAGGTACTGCTCGCGGCTTGTCGCACCGAAAATGTCGATTCCCGCGTTGAGGCGCTT
CAGGGCGCTGGCCTGGAAGAAAAAGTGGTCGATGTCGAAGCTTACGCTATGGAGCGCGCTTTTGAATTGGTGTTGGAGCA
GCTCGAAGATCAGGACAATCAGGTTGTTGCTGTGCTCGATGTCGGCGCCACCATGACCACCCTAAGTGTGCTTGTCGATG
GTCGCACTATCTATACTCGCGAGCAGCTGTTTGGCGGGCGTCAACTCACCGAAGAAATTCAGCGCCGCTACGGTTTGTCG
ATGGAAGAAGCCGGGCTTGCGAAAAAGCAGGGCGGTCTGCCTGATGACTATCAGTCCGAAGTGCTTGAGCCGTTTAAAGA
GGCGGTAATCCAGCAGATTACTCGTTCGCTGCAATTCTTTTTCTCTTCCAGTGAATACAATGATGTGGATTACATCGTCC
TGGCAGGCGGTGTGGCTTCTATGGAAGGCCTGGTTGAATTAATAGAGGAAAAGCTTGGCACGCAAACACTGGTTGCCAAT
CCCTTTGCGAACATGTCGGTCGCATCGAGGGTGAACGCAGCAGCTCTTGCCAATGATGCCCCTTCGCTGATGGTGGCAAC
CGGACTGGCGATGAGGAGCTTTGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-354)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

54.755

97.746

0.535
  comM Acinetobacter nosocomialis M2

54.755

97.746

0.535
  comM Acinetobacter baylyi ADP1

53.602

97.746

0.524
  pilM Legionella pneumophila strain ERS1305867

46.591

99.155

0.462