Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   NG812_RS02195 Genome accession   NZ_CP099987
Coordinates   444496..446706 (+) Length   736 a.a.
NCBI ID   WP_270252680.1    Uniprot ID   -
Organism   Lactococcus garvieae strain FUA009     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 439496..451706
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG812_RS02180 (NG812_02170) - 440871..442427 (+) 1557 WP_004259192.1 ABC-F family ATP-binding cassette domain-containing protein -
  NG812_RS02185 (NG812_02175) - 442593..443768 (+) 1176 WP_004259189.1 SLC13 family permease -
  NG812_RS02190 (NG812_02180) - 443826..444476 (+) 651 WP_019299803.1 helix-hairpin-helix domain-containing protein -
  NG812_RS02195 (NG812_02185) comEC 444496..446706 (+) 2211 WP_270252680.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  NG812_RS02200 (NG812_02190) - 446861..447637 (+) 777 WP_042217333.1 alpha/beta hydrolase family protein -
  NG812_RS02205 (NG812_02195) - 447833..448048 (+) 216 WP_003135074.1 F0F1 ATP synthase subunit C -
  NG812_RS02210 (NG812_02200) atpB 448103..448816 (+) 714 WP_004259176.1 F0F1 ATP synthase subunit A -
  NG812_RS02215 (NG812_02205) atpF 448830..449336 (+) 507 WP_004259173.1 F0F1 ATP synthase subunit B -
  NG812_RS02220 (NG812_02210) - 449351..449878 (+) 528 WP_165705973.1 F0F1 ATP synthase subunit delta -
  NG812_RS02225 (NG812_02215) atpA 449944..451452 (+) 1509 WP_004259170.1 F0F1 ATP synthase subunit alpha -

Sequence

Protein


Download         Length: 736 a.a.        Molecular weight: 85228.88 Da        Isoelectric Point: 9.6535

>NTDB_id=703190 NG812_RS02195 WP_270252680.1 444496..446706(+) (comEC) [Lactococcus garvieae strain FUA009]
MKQKFSLIYFVYLLVFVYFLIFSFSWPLLILSLFSFFIAFYRQYYSVLVLLLCFSLFLLMIKKETEVQERKQPEKISKIT
PYMDTLQVNGSRLSFRGEVNGNDFQAFYTLTSEKEKEYFKKLAVKGTFYFEGTLEIPEEQRNFSGFDYRKYLRTQGVYRL
LKIEKIHGFEEKQDFDLRLVRRKAILWAQEHFPSPMSSYMTGLLFGWLDKDFEEMGELYTSLGIIHLFALSGMQVNFFIE
LLRNILLRLGLSQKLVFFIQIPFSIFYAFLTGLSLSILRALLQKNIPLKNSSDRFSVTFLLLMVFSPYFLLTPGGQLTML
YSFLITFFSRKFKELKGLKKSLLDSTVLAVGVLPLLIFHFHSFQPLSIILTLLFSLIFDFLLLPGLLFVFLLSLFGTALT
LFNPFFIVLESTVRWVDDLFDQPIVFGKPNLIFLLLLFMLSGLALDFYKKRNWNLSIIIVLFLLFFFVKNPKHPSITMVD
VGQGDSILLQDSWNSQNILIDTGGRLKIRADEQWKEGIYKSNAENTLLPYLRARGVSKIDVLIVTHPDEDHIGDLESVAQ
KVPIKNVYVSASALEKKSFAKKLSRLNSQIHIVQEGDKLPIFNSYLWFLTTGVEGKSDNDNSLVTFGEFYGKKFLFTGDL
EEEGEVRLQEEYPHLEVDVLKVGHHGSKTSSKEAFLEAINPKLALISAGKNNRYKHPNQETLQQFAARGIKIYRTDQQGA
IRLLYKKGSWHIQTVK

Nucleotide


Download         Length: 2211 bp        

>NTDB_id=703190 NG812_RS02195 WP_270252680.1 444496..446706(+) (comEC) [Lactococcus garvieae strain FUA009]
ATGAAACAAAAATTTTCGCTTATCTATTTCGTTTATCTCTTAGTCTTTGTTTATTTTCTTATTTTTAGTTTTTCATGGCC
TTTACTTATTCTTAGTCTCTTTAGTTTCTTCATCGCTTTTTATCGTCAATACTATAGTGTGTTAGTTCTTTTACTGTGCT
TTAGTTTATTCTTACTCATGATAAAAAAAGAAACGGAAGTACAAGAAAGAAAGCAACCAGAAAAAATTTCGAAAATTACC
CCTTATATGGATACTCTTCAAGTAAATGGAAGCAGGCTCAGTTTCAGAGGAGAAGTGAACGGCAACGACTTCCAAGCTTT
TTACACCTTGACCTCGGAAAAAGAAAAAGAATACTTCAAAAAACTTGCGGTGAAGGGGACTTTTTACTTTGAAGGCACTT
TGGAGATTCCAGAAGAGCAAAGAAACTTTTCAGGTTTTGATTACAGAAAGTATTTAAGGACTCAAGGGGTCTATCGTCTT
CTTAAAATAGAAAAAATTCATGGCTTTGAAGAAAAACAGGACTTTGATTTACGGCTGGTACGCCGAAAAGCCATTCTTTG
GGCTCAGGAACATTTCCCTTCTCCTATGTCGTCCTATATGACAGGGCTTCTTTTTGGGTGGTTGGATAAAGACTTTGAAG
AAATGGGGGAGCTTTACACTAGCCTTGGTATTATCCATTTGTTTGCGCTGTCAGGCATGCAGGTGAATTTTTTTATTGAA
CTTCTCCGAAATATTTTACTACGGCTTGGCTTGTCACAGAAGTTAGTCTTTTTTATCCAAATTCCCTTTTCGATATTTTA
TGCATTTCTTACAGGCTTATCTCTTTCAATCTTACGCGCGCTTCTTCAAAAAAATATCCCCCTTAAAAATTCAAGCGATA
GATTTTCTGTCACTTTTCTTCTTTTAATGGTGTTCAGTCCTTATTTCCTGCTGACACCAGGAGGACAACTCACGATGCTT
TATTCTTTCTTAATCACATTTTTTTCACGAAAGTTCAAAGAACTAAAAGGATTAAAAAAATCGTTACTTGATTCTACCGT
CCTAGCTGTTGGGGTTCTTCCGCTTTTGATTTTTCACTTCCATAGTTTTCAGCCATTATCGATTATCTTGACTTTATTAT
TTAGTCTGATTTTTGATTTTTTACTTCTGCCAGGTTTACTTTTTGTTTTTCTTCTTTCTTTATTCGGTACTGCTCTCACG
TTATTTAATCCTTTTTTTATTGTTTTAGAATCAACAGTAAGATGGGTGGACGATTTATTTGACCAGCCGATTGTCTTTGG
CAAACCCAACCTTATTTTTTTACTTTTACTTTTTATGCTGAGTGGACTTGCCCTTGATTTTTATAAAAAAAGAAACTGGA
ACTTGAGCATAATAATTGTTCTTTTCTTGCTTTTTTTCTTTGTTAAGAATCCTAAACACCCAAGCATTACAATGGTAGAT
GTGGGACAAGGGGACAGTATTCTTTTACAAGACAGTTGGAACAGCCAAAACATCCTCATCGATACGGGCGGGAGATTAAA
AATAAGAGCTGATGAGCAGTGGAAGGAGGGAATCTATAAGAGCAATGCCGAAAATACACTTCTGCCTTATCTGAGAGCGC
GTGGCGTGAGTAAGATAGATGTTCTGATTGTGACACATCCAGATGAGGATCATATTGGAGACCTGGAGAGTGTAGCTCAG
AAAGTACCAATAAAAAATGTTTATGTGTCAGCTTCTGCATTGGAGAAGAAAAGCTTTGCTAAAAAACTCAGCCGTTTAAA
CAGTCAGATTCACATTGTGCAGGAGGGGGACAAGCTCCCGATCTTTAACAGTTATCTCTGGTTTTTAACGACAGGCGTTG
AAGGGAAAAGCGACAATGATAATTCTTTAGTGACATTTGGTGAATTTTATGGGAAGAAATTTCTTTTTACAGGCGACTTA
GAAGAAGAGGGAGAGGTGCGGCTCCAGGAAGAATATCCCCATTTAGAGGTGGATGTCCTAAAAGTTGGACATCATGGCAG
TAAAACTTCTTCAAAGGAAGCTTTTTTAGAAGCAATCAACCCTAAGCTCGCTCTTATTTCGGCTGGAAAGAATAATCGAT
ATAAACATCCCAATCAAGAAACGCTCCAACAATTCGCTGCACGAGGTATAAAAATTTATCGTACAGACCAGCAAGGGGCA
ATCCGTCTCCTTTATAAAAAAGGCAGTTGGCACATTCAAACAGTAAAGTAG

Domains

Predicted by InterproScan.

Competence protein (Competence)

(203-445)

Metallo-beta-lactamase superfamily (Lactamase_B)

(481-689)

Domain of unknown function (DUF4131) (DUF4131)

(24-166)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Lactococcus lactis subsp. cremoris KW2

53.07

99.592

0.529
  comEC/celB Streptococcus mitis SK321

45.248

100

0.459
  comEC/celB Streptococcus mitis NCTC 12261

43.817

100

0.443
  comEC/celB Streptococcus pneumoniae Rx1

43.24

100

0.439
  comEC/celB Streptococcus pneumoniae D39

43.24

100

0.439
  comEC/celB Streptococcus pneumoniae R6

43.24

100

0.439
  comEC/celB Streptococcus pneumoniae TIGR4

43.338

100

0.438