Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   NG812_RS00850 Genome accession   NZ_CP099987
Coordinates   167841..168860 (+) Length   339 a.a.
NCBI ID   WP_270252906.1    Uniprot ID   A0AAX3NEB9
Organism   Lactococcus garvieae strain FUA009     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 162841..173860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG812_RS00830 (NG812_00830) - 163221..164822 (-) 1602 WP_165705509.1 GH25 family lysozyme -
  NG812_RS00835 (NG812_00835) guaB 164991..166472 (+) 1482 WP_165705510.1 IMP dehydrogenase -
  NG812_RS00840 (NG812_00840) rpsU 166595..166771 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  NG812_RS00845 (NG812_00845) comGA 166958..167902 (+) 945 WP_165705597.1 competence type IV pilus ATPase ComGA Machinery gene
  NG812_RS00850 (NG812_00850) comGB 167841..168860 (+) 1020 WP_270252906.1 competence type IV pilus assembly protein ComGB Machinery gene
  NG812_RS00855 (NG812_00855) comYC 168879..169184 (+) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  NG812_RS00860 comGD 169159..169575 (+) 417 WP_165705512.1 competence type IV pilus minor pilin ComGD -
  NG812_RS00865 comGE 169607..169840 (+) 234 WP_229031266.1 competence type IV pilus minor pilin ComGE -
  NG812_RS00870 (NG812_00865) comGF 169821..170261 (+) 441 WP_165705513.1 competence type IV pilus minor pilin ComGF -
  NG812_RS00875 (NG812_00870) - 170494..171339 (+) 846 WP_165705514.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  NG812_RS00880 (NG812_00875) - 171425..172294 (-) 870 WP_003133725.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39550.25 Da        Isoelectric Point: 9.8215

>NTDB_id=703174 NG812_RS00850 WP_270252906.1 167841..168860(+) (comGB) [Lactococcus garvieae strain FUA009]
MDLSQLLQLRTKKLSLAKQIKLIQLMNNLFKSGFHLSEIIDFLERSGLTEAYFISKMREGLLNGQSFSGILAKLKFNEDI
VTQLSLAESHGNVEYTLGLIEEKLRRVLNIRKKLIQVATYPFVLLVFLIFIMLGLKNYLLPQLDENRGLATYVIQNLPTL
FLGFLFSLLIVFYLGRYYWKKKTALEAMRLMVKIPFVGRFVQLYLTAYFSREWGNLIAQAVDLRQICFIMQEQKSRIFKE
FGFELLHILDRGQKFEDALRFYPIFTKELALIVEYGELKSKLGKELMVFSEESWSLFFERVERAMQLIQPIVFLLVALLI
ILIYAAMLLPIYSNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=703174 NG812_RS00850 WP_270252906.1 167841..168860(+) (comGB) [Lactococcus garvieae strain FUA009]
ATGGATTTATCTCAGCTGTTACAGCTGCGTACGAAAAAATTGAGTTTAGCGAAGCAGATTAAGCTTATTCAACTTATGAA
TAATCTTTTCAAAAGTGGCTTTCACCTTTCTGAAATTATTGATTTTCTTGAACGGTCGGGTCTCACGGAAGCATACTTTA
TTTCCAAAATGCGTGAAGGTTTACTCAACGGGCAAAGTTTCTCTGGTATTTTAGCGAAGCTCAAATTTAATGAGGATATT
GTGACTCAGCTCTCTTTGGCCGAGTCTCATGGGAATGTTGAGTATACTTTAGGGTTGATTGAAGAAAAACTGAGACGCGT
TTTGAACATTCGCAAAAAGCTCATCCAAGTTGCCACTTACCCGTTCGTTCTTCTGGTATTTCTAATCTTTATCATGCTAG
GGTTAAAAAATTATTTACTGCCTCAACTGGACGAAAACAGAGGATTAGCAACTTATGTTATACAGAATCTTCCGACTCTC
TTTTTAGGTTTCCTCTTCAGTTTGCTTATTGTCTTTTATCTGGGCAGATACTATTGGAAGAAAAAAACTGCTTTAGAAGC
GATGAGGCTAATGGTCAAAATACCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACAGCTTATTTTTCAAGAGAATGGG
GAAACTTAATTGCTCAAGCAGTCGATTTACGTCAGATTTGTTTTATTATGCAAGAACAAAAAAGTCGAATCTTCAAAGAA
TTTGGTTTTGAGCTCCTTCATATATTAGATAGGGGTCAGAAGTTTGAAGACGCCCTTCGATTTTACCCCATATTTACCAA
GGAGTTGGCATTAATTGTGGAATATGGTGAACTAAAGTCTAAGCTAGGGAAAGAGTTAATGGTTTTTTCTGAAGAAAGCT
GGTCGCTCTTTTTTGAAAGAGTTGAAAGAGCAATGCAGCTTATCCAGCCTATCGTTTTCTTGCTCGTGGCGCTGTTGATT
ATTTTAATTTATGCTGCAATGTTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

53.571

99.115

0.531
  comGB/cglB Streptococcus mitis NCTC 12261

52.836

98.82

0.522
  comYB Streptococcus gordonii str. Challis substr. CH1

52.381

99.115

0.519
  comGB/cglB Streptococcus mitis SK321

52.239

98.82

0.516
  comGB/cglB Streptococcus pneumoniae R6

51.94

98.82

0.513
  comGB/cglB Streptococcus pneumoniae Rx1

51.94

98.82

0.513
  comGB/cglB Streptococcus pneumoniae D39

51.94

98.82

0.513
  comGB/cglB Streptococcus pneumoniae TIGR4

51.94

98.82

0.513
  comYB Streptococcus mutans UA140

51.429

92.92

0.478
  comYB Streptococcus mutans UA159

51.111

92.92

0.475