Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   NI375_RS13800 Genome accession   NZ_CP099956
Coordinates   3006884..3007477 (-) Length   197 a.a.
NCBI ID   WP_308386895.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain Isc15A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3001884..3012477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI375_RS13780 (NI375_13985) aroB 3002770..3003870 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  NI375_RS13785 (NI375_13990) aroK 3003903..3004421 (-) 519 WP_005381319.1 shikimate kinase AroK -
  NI375_RS13790 (NI375_13995) - 3004609..3006353 (-) 1745 Protein_2656 type IV pilus secretin PilQ -
  NI375_RS13795 (NI375_14000) pilP 3006379..3006894 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  NI375_RS13800 (NI375_14005) pilO 3006884..3007477 (-) 594 WP_308386895.1 type 4a pilus biogenesis protein PilO Machinery gene
  NI375_RS13805 (NI375_14010) pilN 3007470..3008045 (-) 576 WP_104975648.1 PilN domain-containing protein Machinery gene
  NI375_RS13810 (NI375_14015) pilM 3008029..3009045 (-) 1017 WP_005381324.1 type IV pilus biogenesis protein PilM Machinery gene
  NI375_RS13815 (NI375_14020) - 3009190..3011751 (+) 2562 WP_308386896.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23045.54 Da        Isoelectric Point: 5.0885

>NTDB_id=702946 NI375_RS13800 WP_308386895.1 3006884..3007477(-) (pilO) [Vibrio alginolyticus strain Isc15A]
MVDWQDLEIDEMAEWPLLPQIIVIALLIIVIQGLGYWFYLKPKDEQLNHLIQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNEFGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEETKNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=702946 NI375_RS13800 WP_308386895.1 3006884..3007477(-) (pilO) [Vibrio alginolyticus strain Isc15A]
GTGGTTGATTGGCAGGATCTCGAAATCGATGAAATGGCCGAGTGGCCATTGTTGCCCCAGATTATAGTAATCGCGCTTCT
GATCATAGTGATTCAAGGCTTAGGTTATTGGTTTTACCTTAAACCGAAAGACGAACAGTTGAATCACTTGATTCAGCAGG
AGCAGACACTCAAGGTGGCATTGAAGATCAAAGCGAACAAAGTGGCAGCGTTGCCTCAGCTTCAATCTCAATTGGATGAA
CTTACCACTCGTTACGAGTTATTACTGCAACAACTGCCTGCGCAAAAAGAGCTGGCTACAATGCTCGCTTCAGTGAATGA
ATTTGGCATCGACAATAAGCTGACTTTCACCCGAATCGACTGGGGCGAAAAACAATCAGAGCACTTCCTTTATCGTCTCC
CTTTAAACATTGAGTTAACGGGCGACTTTCATGACATCGGCCATTTTTCTCAGGCGATAGCGACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAACGTGTCAGCCAAGAGAGCGAAACTCTGCACTTTCGGGTGCGCGCTAACACCTATCA
GTTTAAGCCGGAGGAGACGAAAAATGACAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

86.316

96.447

0.832
  pilO Vibrio cholerae strain A1552

69.634

96.954

0.675