Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NI375_RS13810 Genome accession   NZ_CP099956
Coordinates   3008029..3009045 (-) Length   338 a.a.
NCBI ID   WP_005381324.1    Uniprot ID   A0AAW9BNQ7
Organism   Vibrio alginolyticus strain Isc15A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3003029..3014045
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI375_RS13785 (NI375_13990) aroK 3003903..3004421 (-) 519 WP_005381319.1 shikimate kinase AroK -
  NI375_RS13790 (NI375_13995) - 3004609..3006353 (-) 1745 Protein_2656 type IV pilus secretin PilQ -
  NI375_RS13795 (NI375_14000) pilP 3006379..3006894 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  NI375_RS13800 (NI375_14005) pilO 3006884..3007477 (-) 594 WP_308386895.1 type 4a pilus biogenesis protein PilO Machinery gene
  NI375_RS13805 (NI375_14010) pilN 3007470..3008045 (-) 576 WP_104975648.1 PilN domain-containing protein Machinery gene
  NI375_RS13810 (NI375_14015) pilM 3008029..3009045 (-) 1017 WP_005381324.1 type IV pilus biogenesis protein PilM Machinery gene
  NI375_RS13815 (NI375_14020) - 3009190..3011751 (+) 2562 WP_308386896.1 PBP1A family penicillin-binding protein -
  NI375_RS13820 (NI375_14025) oxyR 3011805..3012716 (-) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -
  NI375_RS13825 (NI375_14030) - 3012886..3013614 (+) 729 WP_262630461.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37581.92 Da        Isoelectric Point: 7.2224

>NTDB_id=702948 NI375_RS13810 WP_005381324.1 3008029..3009045(-) (pilM) [Vibrio alginolyticus strain Isc15A]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKQIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEESEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEGGQSGSDSYQVFATRKDVVEGRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTLSSSFTMFTEQGELFHKEFAYGTRGANSASPVDFLSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAVDWQHFSTAA
GLAVRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=702948 NI375_RS13810 WP_005381324.1 3008029..3009045(-) (pilM) [Vibrio alginolyticus strain Isc15A]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTCAAGCAGATAAAAAAAGAACTGCCTTTTGGCGCTAAAAAAGTTGCGATCTCAGTTCCTGATAACTCG
GTAATTAGCAAGAAACTGCAAATAGAACAAAATCTTGAAGAGAGTGAAGTTGAGTTTGCAGTGATACAAGCCTTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTTCGTCTTCGCGCTGAGGGCGGGCAGAGTGGCAGTGATA
GCTATCAAGTGTTTGCGACAAGAAAAGATGTGGTAGAAGGCCGAGTCGATGCATTACAAAAGTCCGGACTAAAACCTGTT
TTAGTGGACGTGCATTCGCAAAGCCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAACAAATACTG
CTTACTCGATATTGGCACCTTATCGAGCTCCTTCACCATGTTTACCGAACAAGGTGAACTGTTTCACAAAGAGTTTGCTT
ACGGCACACGCGGAGCAAACAGCGCTTCTCCAGTCGACTTTCTGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAC
CGTCAAATCGTAGAGCGAGTAAAGCGCCAGATGCAGCTCTATACCTCAATCAATGGCTCACAAAACATCAAGGGTATCTG
GTTATCAGGGGAAGGGGCCTCCACTCCCATGCTCGCAGAGGAGTTGTCTCAACAATTGTCGTTAGAGTGTGAATTGCTAA
ATCCGCTAGGGCTATTTGAGATGAAGGTGTCTAAGCGTAAGCGTCGTGCTGTGGATTGGCAGCATTTCTCAACAGCAGCC
GGACTGGCCGTTCGTGGCATTCATTGGCTAGGAGGCGCCCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

83.136

100

0.831
  pilM Vibrio cholerae strain A1552

50.746

99.112

0.503