Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PCP76_RS28570 Genome accession   NZ_CP115297
Coordinates   6083318..6083941 (+) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain 135     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6078318..6088941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP76_RS28555 (PCP76_28535) - 6079005..6081476 (-) 2472 WP_039026606.1 penicillin-binding protein 1A -
  PCP76_RS28560 (PCP76_28540) pilM 6081661..6082725 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  PCP76_RS28565 (PCP76_28545) pilN 6082725..6083321 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  PCP76_RS28570 (PCP76_28550) pilO 6083318..6083941 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  PCP76_RS28575 (PCP76_28555) comL/pilP 6083938..6084462 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  PCP76_RS28580 (PCP76_28560) pilQ 6084516..6086660 (+) 2145 WP_033977030.1 type 4a pilus secretin PilQ Machinery gene
  PCP76_RS28585 (PCP76_28565) aroK 6086672..6087190 (+) 519 WP_003095833.1 shikimate kinase AroK -
  PCP76_RS28590 (PCP76_28570) aroB 6087240..6088346 (+) 1107 WP_073648621.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=702866 PCP76_RS28570 WP_003103268.1 6083318..6083941(+) (pilO) [Pseudomonas aeruginosa strain 135]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=702866 PCP76_RS28570 WP_003103268.1 6083318..6083941(+) (pilO) [Pseudomonas aeruginosa strain 135]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGAGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401